Long-Pulse Gastric Electrical Stimulation Repairs Interstitial Cells of Cajal and Smooth Muscle Cells in the Gastric Antrum of Diabetic Rats

Figure 5

SMC expressed in different groups. (a) Double immunofluorescence showed that after the given gastric electrical stimulation, more c-kit+ cells among a-SMA+ cells were observed, namely, more ICC is distributed in the myenteric layer and the muscle layer (green, a-SMA; red, c-kit; blue, Hoechst 33342; scale bars = 20 μm). (b) Western blot study showed that with long-pulse gastric electrical stimulation, the expression of a-SMA was not significantly changed. (c) RT-PCR showed that the expression of a-SMA mRNA in gastric antrum was not significantly changed in each group.

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