Diminished DEFA6 Expression in Paneth Cells Is Associated with Necrotizing Enterocolitis

Figure 1

Image processing: DAB staining detection. (a) Original image—RGB color. (b) Selection of the mucosa as the region of interest (ROI). (c) IHC Toolbox plugin in ImageJ selects color, representing DAB staining. (d) Conversion of an RGB image to an 8-bit image (the lowest pixel intensity represents the highest DEFA6 expression and vice versa). (e) Inversion of pixel intensity values results in the highest pixel intensity corresponding to the highest DEFA6 expression.

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