Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer

Figure 3

CNN3 is critical for doxorubicin resistance in MKN-28 cells. (a) Viability of two different human gastric cancer cell lines (MKN-28 and MKN-45) after treatment with the indicated concentrations of doxorubicin for 48 h. (b) Viability of control siRNA- and CNN3-specific siRNA-transfected MKN-28 cells after treatment with the indicated concentrations of doxorubicin for 48 h. (c) Representative images for TUNEL staining of CNN3-depleted MKN-28 cells after doxorubicin (Dox) treatment (2 μg/ml) for 24 h. The histogram shows the ratio of TUNEL-positive CNN3-depleted MKN-28 cells after Dox treatment (2 μg/ml) for 24 h. The data shown are expressed as the of three individual experiments, each performed in triplicate. as determined by -test. (d) Representative immunoblot for PARP cleavage in control siRNA- and CNN3-specific siRNA-transfected MKN-28 cells after Dox treatment for 48 h.

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