Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry

Figure 6

Immune cell subsets accumulate in bile from patient with cholangiocarcinoma by flow cytometry. Proportion of immune cells and distribution of immune cells. The immune cells were stained with FITC-conjugated anti-human-CD56, APC-Cy7-conjugated anti-human-CD3, APC-conjugated anti-human-CD16, PE-conjugated anti-human-CD4, Percp-conjugated anti-human-TCRγ/δ, and PE-Cy7-conjugated anti-human-CD8 antibody. The cell positive rates are 64.53%, 55.64%, 58.04%, 63.09%, 60.70%, and 55.77%. The immune cells were stained with FITC-conjugated anti-human-HLA-DR, APC-Cy7-conjugated anti-human-CD11b, APC-conjugated anti-human-Lin, PE-conjugated anti-human-CD33, Percp-conjugated anti-human-CD14, and PE-Cy7-conjugated anti-human-CD15 antibody. The cell positive rates are 64.81%, 61.64%, 58.59%, 63.76%, 61.55%, and 50.58%. The immune cells were stained with FITC-conjugated anti-human-CD45, APC-Cy7-conjugated anti-human-CD11b, APC-conjugated anti-human-CD16, PE-conjugated anti-human-CD66b, Percp-conjugated anti-human-CD14, and PE-Cy7-conjugated anti-human-CD15 antibodies. The cell positive rates are 64.93%, 57.66%, 58.76%, 59.48%, 61.58%, and 46.24%.