Research Article
HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome
Figure 2
HAVCR1 expression was significantly decreased in MKN-45 and AGS cells using siRNA strategy. (a) QRT-PCR was used to assess the HAVCR1 mRNA expression in MKN-45 cells after si-HAVCR1#1 and si-HAVCR1#2 transfection, respectively, versus the si-con group. (b and c) The HAVCR1 protein level was evaluated using MKN-45 cells that were transfected with si-HAVCR1#1 or si-HAVCR1#2. The gray value of the protein bands was quantified. versus the si-con group. (d) QRT-PCR analysis showed the silencing efficiency of si-HAVCR1#1 and si-HAVCR1#2 in AGS cells, versus the si-con group. (e and f) The expression of HAVCR1 in AGS cells was detected by western blot and quantified, versus the si-con group.
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