Peritoneal Metastatic Cancer Stem Cells of Gastric Cancer with Partial Mesenchymal-Epithelial Transition and Enhanced Invasiveness in an Intraperitoneal Transplantation Model

Figure 2

(a, b) In the sequential transplantation, gross anatomy generally showed that transplanted tumors, oval-shaped, with diverse size up to maximal 0.6 cm, were located at the greater omentum and interintestinal space. (c) Those tumors distributed along mesenteric vessel with grain-like appearance. (d) When dissected, the tumor tissue presented creamy white color, irregular shape, and hard texture. (e) Histology by H&E staining (×100) showed that tumor cells were clustered, and nuclei were large and with mitotic appearance. Necrotic regions were observed around tumor cell clusters, and no glandular structure was observed in tumor tissue with poor differentiation. (f) The frozen section under fluorescence microscope (×200) showed the formation of intraperitoneal transplanted tumor from the CSC-hGC^[GFP+LUC] (green: tumor cells; blue: DAPI staining for nuclei).

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