Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1

Figure 3

miR-3200-5p was a target of LINC00324. (a) Starbase predicted a binding site between LINC00324 and miR-3200-5p. (b) MGC803 and MKN-45 cells were transfected with mimics NC, miR-3200-5p mimics, inhibitor NC, or miR-3200-5p inhibitor. vs. blank. (c) Relative luciferase activity in MGC803 and MKN-45 cells was measured by the dual-luciferase reporter assay. vs. mimics NC. (d) MGC803 and MKN-45 cells were transfected with si-NC or si-LINC00324. vs. control. (e) The expression of LINC00324 in tumor tissues and adjacent normal tissues of patients with GC was detected by qRT-PCR. vs. normal tissue. (f) The expression of LINC00324 was negatively correlated with miR-3200-5p. (g) The expression of miR-3200-5p in the human normal gastric epithelial cell line GES-1 and GC cell lines (AGS, MGC803, and MKN-45) was detected by qRT-PCR. vs. GES-1.

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