Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare

Figure 1

Metabolic assessment clinical status. (a) Body weight at 12 weeks and 16 weeks showed a significant gain in total body weight of WD and HFD60 mice compared to that of control animals. (b) Food intake per week for 12 weeks and 16 weeks showed a significantly higher energy intake in HFD60 mice than that in other groups after 12 weeks of diet. (c) Area under the glucose time curve (AUC) after dietary treatment for 12 or 16 weeks. HFD60 and WD animals displayed significantly impaired glucose tolerance, which is depicted as an increase in IP-GTT AUC compared to chow-treated animals. Comparing HFD60 and WD mice, impaired glucose tolerance was more pronounced after 16 weeks in HFD60 mice. (d) HOMA-IR at 12 weeks and 16 weeks displayed insulin resistance in HFD60 mice compared to control mice but no difference between control and WD mice after 12 and 16 weeks. (e) Triglyceride (TAG) and cholesterol levels at 12 weeks and 16 weeks showed increased cholesterol levels in HFD60 and WD mice after 12 and 16 weeks. TAG levels were only increased significantly in HFD60 mice compared to control and WD mice after 16 weeks of diet. (f) Clinical scores for 12-week and 16-week studies display no or only mild impairment with a maximum DS1. Mild impairment was based on fur defects due to fighting animals. After separating the animals, the fur defects ceased. , , , and .

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