Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare

Figure 4

Sirius red and Ly6G staining. Representative histological images of (a, b) control, (c, d) HFD60, and (e, f) WD mice at 16 weeks for Sirius red and 12 weeks for Ly6G. (a, c, and e) Sirius red staining, magnification 200x, scale bars 200 μm, (b, d, and f) Ly6G, magnification 100x, scale bars 100 μm; Sirius red images show fibrosis, especially pronounced in WD (a) and less in HFD60 (c) livers; Ly6G staining reveals clusters of neutrophils in WD-fed mice (b) and more scattered in HFD60 mice (d). Control livers only presented with a few isolated neutrophils.