Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare

Figure 5

Inflammatory response. (a) Ly6G⁺ cells per HPF display a significant difference between WD and control as well as HFD60 and control at 12 weeks and only between HFD60 and control at 16 weeks. (b) F4/80⁺ cells in % of all cells (histopathology) and CD11b⁺F4/80⁺ cells of leukocytes (FACS) at 12 weeks and 16 weeks. A significantly increased proportion of F4/80⁺ cells in histopathological and FACS analyses indicated augmented inflammation and liver injury in WD mice after 12 weeks of diet. Inflammation was also increased in HFD60 but was not as pronounced as that in WD animals. (c) FACS analysis of CD3⁺ NK1.1⁺ cells at 12 weeks and 16 weeks display a switched (d–f) CD4/CD8 ratio in HFD60 and WD livers after 12 and 16 weeks.

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