Immune Modulation Effects of Lactobacillus casei Variety rhamnosus on Enterocytes and Intestinal Stem Cells in a 5-FU-Induced Mucositis Mouse Model

Figure 6

Representative histology of the jejunum showing villus height and crypt depth with hematoxylin and eosin stain. Microscopical findings of the intestinal mucosa from mice exposed to 5-FU-induced mucositis at the jejunum. Goblet cells were found in PAS+AB-stained sections. Intestinal stem cells CD44 markers and proliferation of crypt (Ki67 expression) were shown by IHC methods. CD44 analysis of intestinal stem cells and Ki67 immunolabeling of the jejunum from mice were assessed after 5-FU treatment. 5-FU significantly decreased villus height compared to the saline controls, and the effect was restored by probiotic treatment. 5-FU significantly lengthened the crypt depth of the intestine compared with the saline controls, and the effect was significantly restored by probiotic treatment.