Serum Exosomes Derived from Irritable Bowel Syndrome Patient Increase Cell Permeability via Regulating miR-148b-5p/RGS2 Signaling in Human Colonic Epithelium Cells

Figure 2

Serum exosomes derived from IBS patients (IBS-exos) induce miR-148b-5p upregulation in HT-29 cells. (a) The morphology of serum exosomes derived from IBS patients. (b) Both CD9 and CD63 are positive for IBS-exos and the corresponding control-exos. (c) The PKH67-labelled (green) immature IBS-exos are cocultured with HT-29 cells. Then, the HT-29 cells are fixed and stained with DAPI (blue). The uptake of IBS-exos by HT-29 cells is observed under a confocal microscope. (d) The relative mRNA level of miR-148b-5p is upregulated in the HT-29 cells cocultured with IBS-exo. value < 0.001 vs. Control.

(a)

(b)

(c)

(d)