Serum Exosomes Derived from Irritable Bowel Syndrome Patient Increase Cell Permeability via Regulating miR-148b-5p/RGS2 Signaling in Human Colonic Epithelium Cells

Figure 3

miR-148b-5p inhibitor suppresses the effects of IBS-exo on cell permeability and RGS2 in HT-29 cells. (a) TEER value of the IBS-exo-cultured HT-29 cells transfected with miR-148b-5p inhibitor or miNC. (b) FITC-Dextran influx of the IBS-exo-cultured HT-29 cells transfected with miR-148b-5p inhibitor or miNC. (c) Quantitative PCR is used to examine miR-148b-5p and RGS2 mRNA in HT-29 cells with different treatments as indicated above. (d) RGS2 protein level is detected in HT-29 cells with different treatments. Prior to IBS-exo treatment for 24 h, HT-29 cells are pretransfected with miR-148b-5p inhibitor or miNC. value < 0.001 vs. control, ^!!! value < 0.001 vs. miNC.

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