Serum Exosomes Derived from Irritable Bowel Syndrome Patient Increase Cell Permeability via Regulating miR-148b-5p/RGS2 Signaling in Human Colonic Epithelium Cells

Figure 4

miR-148b-5p inhibits transcription of RGS2 through binding to its 3UTR. (a) Wild type (WT) and mutant (Mut) binding sites in the 3UTR of RGS2 for miR-148b-5p. (b) Luciferase activity of the HT-29 cells with different treatments. HT-29 cells are cotransfected with miR-148b-5p mimic and the luciferase reporter vector containing WT or Mut binding sites. value < 0.001 vs. miNC+WT.

(a)

(b)