FASN Knockdown Inhibited Anoikis Resistance of Gastric Cancer Cells via P-ERK1/2/Bcl-xL Pathway

Figure 3

Small interfering RNA targeting fatty acid synthase (siFASN) promoted apoptosis while suppressing cell aggregation and FASN expression. (a) Relative fatty acid synthase (FASN) mRNA expression levels in MNK-45 and AGS cells in the anoikis resistance (AR), AR+ small interfering RNA negative control (siNC), and AR + siFASN groups were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). (b) Representative pictures of relative FASN protein expression levels in MNK-45 and AGS cells in the AR, AR + siNC, and AR + siFASN groups during western blot. β-Actin was used as a loading control. (c) Relative FASN protein expression levels in MNK-45 and AGS cells in the AR, AR + siNC, and AR + siFASN groups were assessed by western blot. (d) Cell apoptosis was evaluated using a flow cytometer and an Annexin-V-FITC Apoptosis Detection Kit with PI. (e) Quantitation of the apoptosis rates of MNK-45 and AGS cells in the AR, AR + siNC, AR + siFASN groups determined through cell apoptosis assay. (f) Pictures of the morphology of MNK-45 and AGS cells in the AR, AR + siNC, and AR + siFASN groups were taken under a microscope during suspension culture. (g) Numbers of aggregated MNK-45 and AGS cells in the AR, AR + siNC, and AR + siFASN groups were manually counted by microscopy. ^{^^} p < 0.01 vs. AR + siNC group; ^{^^^} p <0.001 vs. AR + siNC group. Data were performed as the means ± standard.

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