mir-182-5p Regulates Cell Growth of Liver Cancer via Targeting RCAN1

Figure 4

miR-182-5p targets RCAN1 in liver cancer cells. (a) The targeted binding sites of miR-182-5p and RCAN1 were predicted via bioinformatics; (b) The luciferase activities of the Hep3b cell line in different transfection groups were measured by dual-luciferase reporter gene assay; (c) the expression of RCAN1 mRNA in different transfection groups of the Hep3b cell line was assessed through qRT-PCR; (d) the protein expression of RCAN1 in Hep3b cell line in various transfection groups was detected by western blot; .

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