mir-182-5p Regulates Cell Growth of Liver Cancer via Targeting RCAN1

Figure 5

miR-182-5p promotes cell progression of liver cancer via targeting RCAN1. (a) The expression levels of RCAN1 mRNA in Hep3b cells in different transfection groups (, , and ) were detected by qRT-PCR; (b) the protein expression of RCAN1 in Hep3b cells in different transfection groups was assessed through western blot; (c) the proliferative ability of Hep3b cells in different transfection groups was measured via CCK-8 assay; (d) the invasive ability of Hep3b cells in different transfection groups was measured by Transwell assay (100×); (e) the migratory ability of Hep3b cells in different transfection groups was assessed via wound healing assay (40×); (f) changes of Hep3b cell cycle in different transfection groups were tested by flow cytometry; .

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