HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling

Figure 2

Silencing UCA1 reduced proliferation and metastasis of CRC cells. (a) Q-PCR analysis of UCA1 siRNA efficiency. (b) SW480 and DLD-1 cell proliferation rates were measured by MTT assay after being transfected with UCA1 siRNA in four time points. (c) The EMT maker expression level was confirmed by q-PCR analysis in SW480 and DLD-1 cells with UCA1 siRNA transfection. (d) The EMT maker protein level was confirmed by Western blot in SW480 and DLD-1 cells with UCA1 siRNA transfection. (e) The migration ability of SW480 and DLD-1 cells was tested by wound healing assay after being treated with UCA1 siRNA. Quantification was presented, scale bars: 100 μm. (f) The invasion ability of SW480 and DLD-1 was confirmed by transwell assay after being transfected with UCA1 siRNA. Quantification was provided and three independent groups were included, scale bars: 20 μm. ; .

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