HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling

Figure 4

UCA1 modulated miR-185/NOTCH3 signaling. (a) Target scheme of miR-185 and NOTCH3 3UTR. The seed sequence of the target was highlighted with red. (b) The expression of NOTCH3 in CRC patients compared to normal tissue was obtained from TCGA-COAD database. (c) The expression of NOTCH3 in different CRC stages was confirmed based on TCGA-COAD database. Stage IV was compared to stage I. (d) The relation between NOTCH3 expression and overall survival rate was analyzed based on the TCGA-COAD cohort. (e, f) NOTCH3 expression was inhibited by miR-185 confirmed by q-PCR analysis and Western blot in SW480 cells. (g) The target of miR-185 and NOTCH3 was confirmed by luciferase assay. (h) The effect of silencing NOTCH3 on miR-185 expression. (i, j) The effect of silencing UCA1 on NOTCH3 expression analyzed by q-PCR and Western blot. (k) SW480 cell migration ability tested by wound healing assay after the indicated different treatments. The quantification was also presented, scale bars: 100 μm. (l) The SW480 cell invasion ability was tested by transwell assay after the indicated different treatments. The quantification was also presented, scale bars: 20 μm. (m) The regulation model of UCA1, miR-185, and NOTCH3. ; .

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