HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling

Figure 5

HES5 was validated as transcription factor of UCA1. (a) JASPAR predicted the putative HES5 binding motif in the human UCA1 promoter. (b) The expression correlation between UCA1 and HES5 expression in CRC tissue obtained from TCGA-COAD database. (c) Ectopic expression of HES5 by pcD-HES5 overexpression plasmid. (d) UCA1 expression was induced by HES5 overexpression confirmed by q-PCR. (e) and (f) The putative binding site of HES5 on UCA1 located in UCA1 promoter region indicated with red. The target was confirmed by luciferase assay. (g) UCA1and HES5 target was validated by q-ChIP analysis. AchR was served as a negative control. (h) miR-185 and NOTCH3 expression was confirmed by q-PCR analysis after ectopically expressed HES5. NOTCH3 expression was also tested by Western blot. (i) Regulation model of HES5, UCA1, miR-185 and NOTCH3 in CRC progression. , , .

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