Table of Contents
Hepatitis Research and Treatment
Volume 2011, Article ID 939148, 6 pages
Research Article

Evolution of Hepatitis B Virus in a Chronic HBV-Infected Patient over 2 Years

Institute of Basic Medicine of the First People's Hospital of Yunnan Province, Center of Clinical Molecular Biology of Yunnan Province, Kunhua Affiliated Hospital of Kunming Medical College, 157 Jinbi Road, Yunnan Province, Kunming 650032, China

Received 30 January 2011; Revised 11 April 2011; Accepted 10 May 2011

Academic Editor: Runu Chakravarty

Copyright © 2011 Tao Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mutations in full-length HBV isolates obtained from a chronic HBV-infected patient were evaluated at three time points: 1 day, 6 months, and 31 months. While 5 nucleotides variation, and an 18 bp deletion of preS1 have been kept in during at least the first two years, C339T mutation occurring in the hydrophilic region of HBsAg and T770C that caused polymerase V560A substitution were the new point mutations found existing in sequenced clones of the 3rd time point. Internal deletion of coding region obviously appeared in the 3rd time point. The splicers included two new 5-splice donors and three new 3-splice acceptors besides the reported donors and acceptors and may have produced presumptive HBV-spliced proteins or truncated preS proteins. ALT, HBeAg and viral DNA load varied during the follow-up years. These data demonstrated the diversity of genomes in HBV-infected patient during evolution. Combined with clinical data, the HBV variants discovered in this patient may contribute to viral persistence of infection or liver pathogenesis.