Abstract
Objective: The objective of this study was to develop a reproducible
method of establishing the concentration of yeast cells per milliliter of solution.Methods: Three methods of determining the number of yeast cells in
solution were compared: Neubauer's counting chamber, spectrophotometry, and
nephelometry.Results: All three methods were comparable and reproducible.
The following formulas were highly effective in determining the number of yeast cells in
solution: chamber (×