Table of Contents Author Guidelines Submit a Manuscript
Infectious Diseases in Obstetrics and Gynecology
Volume 1, Issue 3, Pages 134-136
http://dx.doi.org/10.1155/S1064744993000304
Basic Science Article

Evaluating the Concentration of a Candida albicans Suspension

1Department of Microbiology, Porto Faculty of Medicine, University of Porto, Porto, Portugal
2Department of Gynecology, Porto Faculty of Medicine, University of Porto, Porto, Portugal
3Department of Microbiology, Porto School of Medicine, Alameda Prof. Hernani Monteiro, Porto 4200, Portugal

Received 2 April 1993; Accepted 25 August 1993

Copyright © 1993 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: The objective of this study was to develop a reproducible method of establishing the concentration of yeast cells per milliliter of solution.

Methods: Three methods of determining the number of yeast cells in solution were compared: Neubauer's counting chamber, spectrophotometry, and nephelometry.

Results: All three methods were comparable and reproducible. The following formulas were highly effective in determining the number of yeast cells in solution: chamber (× 103/ ml) 64.3 + 8,206 × spectrophotometry (absorbance); and chamber (× 103 /ml) –0.2 + 64 × nephelometry (volt).

Conclusions: Utilization of spectrophotometry or nephelometry and the appropriate formula allow for the precise determination, which is easily reproducible, of the concentration of yeast cells in solution. This will facilitate experimentation involving precise inocula or requirement for specific concentrations of yeast cells for various experiments.