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Infectious Diseases in Obstetrics and Gynecology
Volume 3, Issue 2, Pages 73-78
http://dx.doi.org/10.1155/S1064744995000354

Antifungal Effect of Hydrogen Peroxide on Catalase-Producing Strains of Candida spp.

1Department of Obstetrics and Gynecology, Marshall University School of Medicine, Huntington, WV, USA
2Department of Microbiology, Immunology, and Molecular Genetics, Marshall University School of Medicine, 1801 6th Avenue, Huntington 25703, WV, USA

Received 23 January 1995; Accepted 5 June 1995

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: Clinical isolates of Candida were tested for the presence of catalase and susceptibility to hydrogen peroxide.

Methods: MIC was tested by broth dilution technique and catalase was determined by a spectrophotometric procedure.

Results: All 38 strains tested were inhibited by hydrogen peroxide in concentrations ranging from 4.4 to 88 mM/l, with non-albicans isolates generally requiring higher concentrations of hydrogen peroxide for inhibition. Growth media consisting of glucose and protein diminished the antifungal effectiveness of hydrogen peroxide, as did the presence of hemoglobin, in incubation mixtures. However, hydrogen peroxide exerted greater inhibition at pH 4 than at pH 7. Although all Candida isolates tested possessed catalase, there was no apparent correlation between the catalase activity of individual isolates and the minimal antifungal concentration of hydrogen peroxide.

Conclusions: This study suggested that, despite the production of catalase by vaginal microorganisms, hydrogen peroxide may exert a regulating influence which may be further modified by the proteins found in the vaginal milieu.