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Infectious Diseases in Obstetrics and Gynecology
Volume 6 (1998), Issue 2, Pages 61-65
Research Article

Studies on the Pathogenicity of Anaerobes, Especially Prevotella bivia, in a Rat Pyometra Model

1Department of Obstetrics and Gynecology, School of Medicine, Gifu University. 40, Tsukasa-machi, Gifu-city, Gifu 500-8705, Japan
2Institute of Anaerobic Bacteriology, School of Medicine, Gifu University, Gifu, Japan
3Gifu College of Medical Technology, Seki city, Gifu, Japan

Received 26 October 1995; Accepted 11 May 1998

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective: Prevotella bivia is one of the anaerobic bacteria that resides in the flora of the female genital tract. We studied the pathogenicity of P. bivia in a rat pyometra model.

Methods: The experimental animial (rat) model of pyometra was developed to investigate the pathogenicity of P. bivia in a rat pyometra model.

Results: In the groups inoculated with aerobes alone, the infection rate was 10% (1/10) in the Staphylococcus aureus- or Staphylococcus agalactiae -inoculated group and 20% (2/10) in the Escherichia coli-inoculated group. Infection Was not established in the groups inoculated with anaerobes alone. High infection rates were observed in all the mixed-infection groups. In the S. agalactiae- and Bacteroides fragilis-, S. agalactiae- and P. bivia -, E. coli- and B. fragilis-, and E. coli- and P. biviai- inoculated groups, an infection rate of 100% (10/10) was demonstrated. The efficacy of antibiotics such as flomoxef (FMOX) could be determined using a rat pyometra model. In relation to the alteration of vaginal microbial flora during the menstrual cycle, estrogen increased the growth of P. bivia.

Conclusion: Mixture of aerobic bacteria and P. bivia increased the pathogenicity of P. bivia. Estrogen would be useful for raising up the inflammatory change of the uterus in experimental models of genital tract infection due to P. bivia.