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Infectious Diseases in Obstetrics and Gynecology
Volume 2012, Article ID 636474, 14 pages
Research Article

Probiotic Interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the Opportunistic Fungal Pathogen Candida albicans

1Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, Tulsa, OK 74107, USA
2Department of Cell and Tissue Biology, University of California, San Francisco, CA 94143, USA
3Canadian R&D Centre for Probiotics, Lawson Health Research Institute, London, ON, Canada N6A 4V2
4Departments of Microbiology and Immunology, and Surgery, University of Western Ontario, London, ON, Canada N6A 3K7

Received 2 October 2011; Revised 16 February 2012; Accepted 21 February 2012

Academic Editor: Susan Cu-Uvin

Copyright © 2012 Gerwald A. Köhler et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Candida albicans is the most important Candida species causing vulvovaginal candidiasis (VVC). VVC has significant medical and economical impact on women’s health and wellbeing. While current antifungal treatment is reasonably effective, supportive and preventive measures such as application of probiotics are required to reduce the incidence of VVC. We investigated the potential of the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 towards control of C. albicans. In vitro experiments demonstrated that lactic acid at low pH plays a major role in suppressing fungal growth. Viability staining following cocultures with lactobacilli revealed that C. albicans cells lost metabolic activity and eventually were killed. Transcriptome analyses showed increased expression of stress-related genes and lower expression of genes involved in fluconazole resistance, which might explain the increased eradication of Candida in a previous clinical study on conjoint probiotic therapy. Our results provide insights on the impact of probiotics on C. albicans survival.