Probiotic Interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the Opportunistic Fungal Pathogen Candida albicans
Figure 5
Lactobacillus-C. albicans cocultures for transcriptome analysis. The experimental setup employing a 0.45 μm membrane for separation of the bacterial and fungal cultures in 6-well tissue culture plates is represented schematically in (a). Results of a typical Lactobacillus-C. albicans coculture and C. albicans-only control experiment are shown in (b). Growth of C. albicans and Lactobacillus (Lb) in the respective compartments was determined by readings (left -axis). The pH of the culture media is depicted in dashed lines using the right -axis. For transcriptome analysis, total RNA was isolated from C. albicans cells harvested from cocultures and controls.
