Vaginal Colonization by papG Allele II⁺ Escherichia coli Isolates from Pregnant and Nonpregnant Women as Predisposing Factor to Pyelonephritis

Table 3

Nucleotide sequences of PCR primers used to amplify five ExPEC VFs.

Gene	Primer sequence (5′-3′)	Amplicon size (bp)	Reference
hly
F	AACAAGGATAAGCACTGTTCTGGCT	1,177	[18]
R	ACCATATAAGCGGTCATTCCCGTCA
papC
F	GACGGCTGTACTGCAGGGTGTGGCG	328	[22]
R	ATATCCTTTCTGCAGGGATGCAATA
sfa/foc
F	CTCCGGAGAACTGGGTGCATCTTAC	410	[22]
R	CGGAGGAGTAATTACAAACCTGGCA
iucC
F	AAACCTGGCTTACGCAACTGT	269	[23]
R	ACCCGTCTGCAAATCATGGAT
fimH
F	TGCAGAACGGATAAGCCGTGG	508	[24]
R	GCAGTCACCTGCCCTCCGGTA

hly: alpha-hemolysin; papC: P fimbriae; sfa/foc: S fimbriae and F1C fimbriae; iucC: aerobactin; and fimH: type 1 fimbriae.