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Infectious Diseases in Obstetrics and Gynecology
Volume 2016 (2016), Article ID 5293034, 7 pages
http://dx.doi.org/10.1155/2016/5293034
Research Article

Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility

1The Woman’s Hospital of Texas, 7400 Fannin Suite 930, Houston, TX 77054, USA
2The University of Texas Health Science Center at Houston, Medical College, Houston, TX 77054, USA

Received 13 May 2016; Accepted 10 August 2016

Academic Editor: Bryan Larsen

Copyright © 2016 Jonathan Faro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Elucidation of a pathogen’s antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings. Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 105 bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus). Sensitivity was increased to 10−2 bacteria/mL for GBS, 10−1 bacteria/mL for E. faecalis, and 101 bacteria/mL for N. gonorrhoeae following 18–24-hour culture. Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.