Genotypic Variation in Trichomonas vaginalis Detected in South African Pregnant Women

Table 3

Fragment sizes obtained after digestion of the actin gene with the restriction enzymes HindII, MseI, and RsaI as well as the assignment of the T. vaginalis genotypes based on combining the patterns across the three enzyme profiles.

Sample name	HindII fragment sizes	HindII pattern	MseI fragment sizes	MseI pattern	RsaI fragment sizes	RsaI pattern	Genotype
TV4	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV9	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV48	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV79	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV95	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV128	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV184	60, 200 386, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV211	60, 80, 120, 200 330, 426	2	519, 581	1	106, 190, 236, 452	3	I
TV230	60, 200, 426, 449	2	519, 581	1	106, 190, 236, 568	1	G
TV232	60, 200 399, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV253	60, 208 397, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV266	60, 200 386, 426	2	-		106, 236, 568	2	Could not be assigned
TV270	60, 202 387, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV302	60, 200 390, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV357	60, 201 387, 426	2	519, 581	1	106, 190, 236, 568	1	G
TV358	60, 199 388, 426	2	519, 581	1	106, 236, 568	2	H

(-) symbol indicates that no banding pattern was observed. ^#Genotype could not be assigned due to no banding pattern for MseI.