Sugar Variation in Soybean Seed Assessed with a Rapid Extraction and Quantification Method
Table 1
List of soybean genotypes used in this study and
detected mean sugar content.
Genotype
Species
Types and uses
Sugar content
PI 243545
G. max
High-total sugar
105.48
14.23
27.85
0.95
0.25
148.76
03CB-14
G. max
Low oligosaccharide
88.09
3.76
4.14
1.33
0.95
98.27
5002T
G. max
Regular cultivar
77.02
12.68
39.95
1.55
0.33
131.53
Jack
G. max
Regular cultivar
64.37
10.58
37.37
0.68
0.25
113.25
PI 504510
G. max
Regular germplasm
63.02
5.9
36.7
0.8
0.3
106.72
Ozark
G. max
Regular cultivar
58.02
12.09
33.44
1.25
0.4
105.2
SS516
G. max
Small-seeded
56.87
7.1
44.98
1.18
0.25
110.13
CX1834-1-2
G. max
Low phytic acid
56.09
9.9
40.48
0.85
0.23
107.55
R95-1705
G. max
High protein
55.14
3.45
37.29
1.87
1.3
99.05
V97-3000
G. max
Small-seeded
53.94
6.48
43.68
1.48
0.73
106.31
MFS-591
G. max
Small-seeded
52.47
5.38
49.95
1.18
0.2
109.18
Camp
G. max
Small-seeded
52.04
10.7
38.68
0.38
0.13
101.93
PI 323556
G. max
Regular germplasm
43.7
9.47
34.53
1.33
0.37
89.4
MFL-552
G. max
Large-seeded
43.56
15.5
39.5
1.58
0.25
100.39
S02-529
G. max
Regular germplasm
40.14
12.73
37.03
1.68
0.9
92.48
MFL-159
G. max
Large-seeded
31.68
19.73
36.88
1.63
0.13
90.05
PI 200508
G. max
Low oligosaccharide
30.71
0.85
5.15
0.3
0.2
37.21
PI 417559
G. max
Regular germplasm
29.82
5.93
24.5
16.8
12.27
89.32
PI 326581
G. soja
Wild species
22.14
4.05
30.1
1.83
1.0
59.12
PI 153292
G. gracilis
Wild species
61.01
7.85
41.7
1.45
0.23
112.24
sugar melibiose was added in each
sample as internal standard. External sugar standards
at concentrations of 0.5, 1.0, 2.0, 5.0, 10.0, 15.0, and 20.0 μg/600 L were prepared for creating calibration
curves for each of the major sugars. The concentration of each sugar was
estimated by regression of concentration and absorption of individual standard
sugars; ;
;
; ; ; .