Research Article

Sugar Variation in Soybean Seed Assessed with a Rapid Extraction and Quantification Method

Table 1

List of soybean genotypes used in this study and detected mean sugar content.

Genotype Species Types and usesSugar content

PI 243545G. maxHigh-total sugar105.4814.2327.850.950.25148.76
03CB-14G. maxLow oligosaccharide88.093.764.141.330.9598.27
5002TG. maxRegular cultivar77.0212.6839.951.550.33131.53
JackG. maxRegular cultivar64.3710.5837.370.680.25113.25
PI 504510G. maxRegular germplasm63.025.936.70.80.3106.72
OzarkG. maxRegular cultivar58.0212.0933.441.250.4105.2
SS516G. maxSmall-seeded56.877.144.981.180.25110.13
CX1834-1-2G. maxLow phytic acid56.099.940.480.850.23107.55
R95-1705G. maxHigh protein55.143.4537.291.871.399.05
V97-3000G. maxSmall-seeded53.946.4843.681.480.73106.31
MFS-591G. maxSmall-seeded52.475.3849.951.180.2109.18
CampG. maxSmall-seeded52.0410.738.680.380.13101.93
PI 323556G. maxRegular germplasm43.79.4734.531.330.3789.4
MFL-552G. maxLarge-seeded43.5615.539.51.580.25100.39
S02-529G. maxRegular germplasm40.1412.7337.031.680.992.48
MFL-159G. maxLarge-seeded31.6819.7336.881.630.1390.05
PI 200508G. maxLow oligosaccharide30.710.855.150.30.237.21
PI 417559G. maxRegular germplasm29.825.9324.516.812.2789.32
PI 326581G. sojaWild species22.144.0530.11.831.059.12
PI 153292G. gracilisWild species61.017.8541.71.450.23112.24

sugar melibiose was added in each sample as internal standard. External sugar standards at concentrations of 0.5, 1.0, 2.0, 5.0, 10.0, 15.0, and 20.0 μg/600  L were prepared for creating calibration curves for each of the major sugars. The concentration of each sugar was estimated by regression of concentration and absorption of individual standard sugars; ; ; ; ; ; .