Sugar Variation in Soybean Seed Assessed with a Rapid Extraction and Quantification Method

Table 2

Analyses of variance for five major sugar and total sugar contents in twenty soybean genotypes.

Sugar/source^(a)	DF^(b)	Mean square	𝐹 value	Pr>𝐹
Sucrose
Genotype	19	1188.80	25.55	<.0001
Extraction	1	69.80	1.50	.2284
Genotype x extraction	19	61.69	1.33	.2258
Stachyose
Genotype	19	535.99	51.04	<.0001
Extraction	1	0.07	0.01	.9950
Genotype x extraction	19	13.98	1.33	.2227
Raffinose
Genotype	19	87.52	82.00	<.0001
Extraction	1	0.26	0.24	.6265
Genotype x extraction	19	1.56	1.46	.1572
Glucose
Genotype	19	37.58	303.28	<.0001
Extraction	1	1.72	13.88	.0006
Genotype x extraction	19	3.52	28.39	<.0001
Fructose
Genotype	19	21.67	290.03	<.0001
Extraction	1	0.53	7.11	.0114
Genotype x extraction	19	0.24	3.33	.0009
Total sugar
Genotype	19	2102.63	43.72	<.0001
Extraction	1	61.63	1.28	.2649
Genotype x extraction	19	3204.59	3.51	.0005

^(a)Two duplicate extractions following Giannoccaro et al. [16] were prepared from each sample, and each extraction was analyzed in two replicates; ^(b)degree of freedom.