Changes in Protein, Nonnutritional Factors, and Antioxidant Capacity during Germination of L. campestris Seeds

Table 2

Phenolic compounds in L. campestris germinated seeds by HPLC.

Peak number	Retention; time (min)	Phenolic concentration, days of germination^1
		0	1	2	3	4	5	6	7	8	9
1	1.590	145.8	47.3	105.8	100.4	325.0	235.1	444.7	568.9	573.6	452.1
2	1.654	124.4	ND	76.4	118.4	238.9	252.1	347.0	499.2	498.0	408.9
3	1.764	362.8	ND	ND	ND	233.2	ND	231.0	306.8	310.7	272.5
4	7.454	524.7	133.6	149.7	108.3	321.6	137.2	357.7	474.2	482.8	431.3
5	8.941	665.0	ND	243.7	209.4	427.0	332.9	551.5	982.4	837.3	763.1
6	9.169	593.5	215.1	165.0	119.4	380.6	273.0	511.8	931.7	829.9	746.9
7	11.277	ND	ND	ND	ND	ND	111.4	ND	454.8	393.5	ND
8	12.038	ND	ND	ND	ND	ND	272.1	440.4	1270.5	1159.9	853.0
9	12.178	ND	ND	ND	ND	ND	162.2	257.3	696.4	785.6	561.1
10	12.626	395.7	64.5	98.8	ND	ND	134.7	288.5	451.1	400.7	341.3
11	14.455	ND	ND	ND	ND	ND	ND	214.6	354.0	308.4	265.1
12	15.953	ND	ND	ND	99.5	451.2	231.0	701.8	1157.9	668.6	452.8
13	25.195	ND	ND	ND	ND	212.4	87.0	318.9	262.2	253.2	209.4
14	25.361	ND	ND	ND	ND	85.4	ND	62.6	55.9	98.5	90.3
15	25.522	ND	ND	ND	ND	137.8	54.4	255.1	199.0	164.1	128.5
Total concentration		2811.9	460.5	839.5	755.4	2812.9	2282.9	4983.0	8665.1	7764.6	5976.3

¹Phenolic concentration expressed as μg catechin equivalent per g of dry sample.
ND: not determined, under the detection limit.