Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum)
Table 1
Different callus inductions and regenerations media compositions.
Sr. number
Callus induction media
Regeneration media
Rooting media
MS salt (g/L)
Sucrose (g/L)
2, 4-D (mg/L)
MS salt (g/l)
Sucrose (g/l)
NAA (mg/l)
BAP (mg/l)
MS salt (g/l)
Sucrose (g/l)
NAA (mg/l)
1
4.60
20.00
1.00
4.60
20.00
0.50
1.00
4.60
20.00
0.50
2
4.60
30.00
1.00
4.60
20.00
0.50
2.00
4.60
20.00
1.00
3
4.60
40.00
1.00
4.60
20.00
0.50
3.00
4.60
20.00
1.50
4
4.60
20.00
2.00
4.60
20.00
1.00
1.00
4.60
30.00
0.50
5
4.60
30.00
2.00
4.60
20.00
1.00
2.00
4.60
30.00
1.00
6
4.60
40.00
2.00
4.60
20.00
1.00
3.00
4.60
30.00
1.50
7
4.60
20.00
3.00
4.60
20.00
1.50
1.00
4.60
40.00
0.50
8
4.60
30.00
3.00
4.60
20.00
1.50
2.00
4.60
40.00
1.00
9
4.60
40.00
3.00
4.60
20.00
1.50
3.00
4.60
40.00
1.50
10
4.60
30.00
0.50
1.00
11
4.60
30.00
0.50
2.00
12
4.60
30.00
0.50
3.00
13
4.60
30.00
1.00
1.00
14
4.60
30.00
1.00
2.00
15
4.60
30.00
1.00
3.00
16
4.60
30.00
1.50
1.00
17
4.60
30.00
1.50
2.00
18
4.60
30.00
1.50
3.00
19
4.60
40.00
0.50
1.00
20
4.60
40.00
0.50
2.00
21
4.60
40.00
0.50
3.00
22
4.60
40.00
1.00
1.00
23
4.60
40.00
1.00
2.00
24
4.60
40.00
1.00
3.00
25
4.60
40.00
1.50
1.00
26
4.60
40.00
1.50
2.00
27
4.60
40.00
1.50
3.00
5 ml iron (prepared by adding 1000 mg/L myoinositol, 500 mg/L nicotinic acid, 500 mg/L pyridoxine, and 100 mg/L thiamine) and 5 ml vitamin (prepared by adding 0.430 g iron and volume made up to 1 L) solution were also added in the above media.
