Research Article

Phenetic Analysis of Cultivated Black Pepper (Piper nigrum L.) in Malaysia

Table 1

Morphological characteristics used in the phenetic analysis of black pepper cultivars.

Morphological characteristicsMeasurement methods

Leaf characteristics
(1) Leaf shape, leaf apex, and leaf baseDescription based on the UPOV standard
(2) Leaf area (cm2), blade width (, mm), blade length (L, mm), and blade length-width ratio (Lw−1)Measured by using the WinFOLIA image analysis system
(3) Leaf colour (fully expanded leaf)RHS colour codes used

Inflorescence characteristics
(1) Inflorescence length at the stigma withering stage (cm) and inflorescence thickness at the stigma withering stage (mm)Measured by using a vernier calliper
(2) Inflorescence colourRHS colour codes used
(3) Number of flowers per inflorescenceCounted via stereomicroscope
(4) Number of inflorescences (spikes) per branch per nodeCounted manually

Fruit characteristics
(1) Fruit spike length (cm) and fruit size in diameter (mm)Measured by using a vernier calliper
(2) Fruit weight (single fresh berry) (g)Measured by using an analytical balance
(3) Fruit colour (hard dough stage)RHS colour codes used
(4) Percent fruit setCounted manually. Percent = (number of developed fruits)/(number of developed fruits + number of underdeveloped fruits) × 100
(5) Conversion rate (fresh to black pepper) (%)Measured by using an analytical balance (drying specification: oven-drying at 40°C; moisture content ≤12%)
(6) Conversion rate (fresh to white pepper) (%)Measured by using an analytical balance (drying specification: oven-drying at 40°C; moisture content ≤12%)
(7) Pericarp thickness (mm)Measured by using a vernier calliper (horizontal diameter of a fresh berry−horizontal diameter of the seed)

Seed characteristics
(1) Seed diameter (mm)Measured by using a vernier calliper (horizontal diameter of the seed)
(2) Seed weight (g)Measured by using an analytical balance

Vigour
(1) Branch columnBy observation
(2) Internode length (cm)Measurement by using a ruler (node-to-node distance)
(3) Number of nodes/foot of the stemCounted manually

Shoot tips
(1) Anthocyanin: absent or presentBy observation of shoot tip colouration. Green colour = absence of anthocyanin; purple colour = presence of anthocyanin

Withering stage [6].