Research Article | Open Access
Variation and Relationships among Agronomic Traits in Durum Wheat [Triticum turgidum (L.) Thell. ssp. turgidum conv. durum (Desf.) MacKey] under South Mediterranean Growth Conditions: Stepwise and Path Analyses
Relationships among agronomic traits and grain yield were investigated in 56 genotypes of durum wheat (Triticum durum Desf.). The results indicated the presence of sufficient variability nearly for all measured traits. Heritability and expected genetic gain varied among traits. Aboveground biomass, harvest index, and spike number were the most grain yield-influencing traits. Early genotypes showed above-average grain and biological yields, spike number, and lower canopy temperature. Assessed genotypes were clustered into three groups which differed mainly for biological, economical, straw, and grain yields, on the one hand, and plant height, chlorophyll content, and canopy temperature, on the other hand. Selection for direct use from clusters carrying best combinations of yield-related traits and crosses to be made between genotypes belonging to contrasted clusters were suggested to generate more variability. Selection preferentially for spike number, biological yield, harvest index, and canopy temperature to accumulate favorable alleles in the selected entries for future uses is suggested.
Durum wheat (Triticum turgidum L. var. durum) is grown on around 17 million hectares worldwide, representing 8% of the world’s wheat area . It is among the most cultivated crops in the Mediterranean basin, where approximately 75% of the world's durum wheat area is still grown, which contributes to 50% of the worldwide production . In the Mediterranean basin (characterized by highly variable environments), wheat is mainly grown under rainfed conditions, and yield is often constrained by water and heat stresses that are common during the grain-filling period, which is due to the low and unpredictable seasonal rainfalls and high temperatures during the last stages of its development cycle . Terminal drought and heat stresses negatively affect wheat grain weight and yield [4, 5].
Breeders, all over the world, are traying through wheat breeding programs to develop productive and adapted cultivars to their specific environments. This was resulted in the release of several commercial varieties. However, obtaining high-yielding genotypes of durum wheat, especially for Mediterranean rainfed areas, is considered difficult due to high genotype × environment interaction [6, 7]. In Mediterranean rainfed areas, genetic improvement of durum wheat for drought and heat stresses can be achieved by direct or indirect selection for yield. Direct selection involves selecting for yield, whereas indirect selection, that is, physiological breeding, involves selecting for yield components or other associated traits.
Information on the extent of variation and character association is useful for selection purposes to improve durum wheat (Triticum durum Desf.) grain yield. Evaluation of breeding populations is made to determine variability, character association, heritability, and genetic advance, which are needed to measure the genetic gain that can be expected through selection for a given trait. Wheat grain yield improvement was achieved through selection of closely yield-related characters. Sign and strength of correlations of these traits, with each other and with grain yield, depend on the type of plant material assessed and the environment test. It is, therefore, useful to target traits having the greatest influence on grain yield, to maximize yield genetic gain.
Yield components are agronomic traits that provide an opportunity to improve yield under water stress, along with morphological and physiological traits. Among the phonological, morphological, and physiological traits reported in the literature with high heritability and used in breeding programs, we discuss the following ones.
Early flowering, which positively affects yield, provides the ability to escape to terminal drought stress. The reduced number of days to heading and also days to maturity are important when breeding for terminal drought stress tolerance since they allow for drought escape [2, 8–11].
Plant heights, which have permitted the important improvements in grain yield in wheat in the last years, according to Fisher , have been achieved through the deployment of major genes such as the Rht reducing plant height genes (dwarf and semidwarf, the main drivers of the Green Revolution). However, there is a minimum height below which yield limitation is evident . In addition, it has been found that greater plant height has a positive effect on yield in dry Mediterranean rainfed conditions; for this reason, it is desirable that the genotypes maintain their height in arid rainfed areas , compared to irrigated areas . However, plant cycle and size should not be too short and too small since such traits will compromise yields under both stressed and optimum conditions. This may be attributed to low capacity to accumulate sufficient stem reserves for subsequent partitioning to the grain.
Hence, high yield under stressed conditions of some earliest and shortest genotypes is due to their ability to maintain a high number of productive tillers and a relatively high thousand kernel weight. This could have resulted from a lengthy grain-filling period [14, 15].
Drought increases senescence, by accelerating chlorophyll degradation, leading to a decrease in leaf area and canopy photosynthesis. There is evidence that stay-green phenotypes with delayed leaf senescence can improve their performance under drought conditions .
In wheat, leaf chlorophyll content (as a proxy of leaf photosynthesis) and stomatal conductance were proposed as proper criteria for identifying drought-tolerant genotypes under field conditions . These tow traits allow as well as the identification of germplasm combining improved water use efficiency and productivity under both well-watered or water-limited conditions .
Canopy temperature can be used as a tool to indirectly evaluate the transpiration rate of a plant . Olivares-Villegas et al.  reported that, under drought, canopy temperature is the most drought-adaptive trait contributing to a higher performance. This trait is highly heritable and consistently associated with yield phenotypically and genetically. It can be utilized as a selection criterion to identify high-yielding wheat genotypes or as an important predictor of yield performance under drought since it epitomizes a mechanism of dehydration avoidance.
However, not all studies have demonstrated correlated benefits of canopy temperature and grain yield in wheat . Further, the study of Giunta et al.  on durum wheat has indicated that genotypes with warmer canopy temperature, implying more closed stomata, produced the highest yields. Rebetzke et al.  found that preflowering canopy temperature increases were associated with reduced final plant height and increased harvest index and grain yield but were uncorrelated with aerial biomass. In contrast, postflowering, cooler canopies were associated with greater aerial biomass and increased grain number and yield.
Grain yield of cereals is the result of the development and growth of yield components during the life of the crop. The most popular approach to attempt understanding wheat yield is that dividing it into numerical components (i.e., plants per unit land area, spikes per plant, spikelets per spike, grains per spikelet, resulting in the number of grains per m2, and average individual grain weight). This approach is simple to understand and easy to do. It only requires one sample at maturity and a minimum process of the sample to divide the components in the sample, and by multiplying these components, one can obtain the yield . Grain number and grain weight are the main components affecting grain yield and are negatively correlated [23–25]. Graziani et al.  reported that, among yield components, number of grains m−2 showed the strongest correlation with grain yield.
In his study, Mohsin et al.  found that grain yield correlated positively with flag leaf area, plant height, biomass, number of spikes, spike length, number of spikelets per spike, number of grains per spike, 1000-grain weight, and harvest index.
Fellahi et al.  reported for bread wheat a positive and significant correlation of seed yield with biological yield, spike weight, and spike number. Aboveground biomass had the highest effect, explaining more than 83.0% of grain yield variation. This suggested that any increase in biological yield, particularly the spike weight fraction, affected positively grain yield. Aboveground biomass was largely determined by spike number, which explained 85.20% of total variation in biological yield. Kandel et al.  reported similar results for these traits. In addition, moderate to high positive and significant correlations of grain yield with number of spikes, grains per spike, and 1000-grain weight under both stressed and optimum conditions imply the direct contribution of these yield components to yield and should be considered as important target traits during selection [14, 15], and it can be confirmed that maintenance of a high number of productive tillers and kernels per spike contributes more to the grain yield when compared to the other yield components under stress because the number of grains produced per plant will compensate better for the reduction in seed weight . However, under optimum conditions, all the yield components have considerable contribution to grain yield, implying that selection for any of the yield components could significantly improve the yields . Straw yield was positively related to spike number while thousand kernel weight and plant height exhibited significant and negative correlations with seed number per spike . This suggested that compensation effect was operating among traits, lessening the overall effect on grain yield. Fellahi et al.  reported that any increase in spike number improved both aboveground biomass and grain yield. As selection criteria, spike number could be easily appreciated visually, in the field. Furthermore, according to Royo et al. , grain yield increases have been mostly based on increases in harvest index and number of grains per unit area through an enhanced number of spikes and grain set.
Salmi et al.  found appreciable variability for several durum wheat traits such as days to heading, plant height, aboveground biomass, grain yield, and yield components. Broad-sense heritability was high for days to heading and plant height, intermediate for spike number and number of kernels per spike, and low for grain yield and aboveground biomass.
On the other hand, Graziani et al.  found high broad-sense heritability for yield, in contrast with González-Ribot et al. . For the yield components (spike number, grain number, and grain number per spike) and harvest index, broad-sense heritability was low to moderate [2, 25]. Mohsin et al.  and Arya et al.  found high broad-sense heritability for harvest index. Heritability was, respectively, high to moderate for the physiological traits chlorophyll content and canopy temperature [2, 10].
Expected genetic gain, expressed as % of trait mean, varied from 4.8% for days to head to 45.9% for grain yield. Relatively to check the cultivar, the best selected entries, for the grain yield, were taller and had an above average biological yield; the harvest index and spike number were below the average thousand kernel weight . In the same context, Arya et al.  found the lowest value for days to maturity (9.44%) while the highest gain for number of grains per spike (36.88%). For grain yield, expected genetic gain was 31.98%.
Hannachi et al.  reported that both additive and dominance gene actions were implicated in the expression of durum wheat traits, H1 component was larger than D component for days to heading, spike number, kernel per spike, and grain yield and smaller for plant height and thousand kernel weight. This indicated the complex inheritance pattern of yield and yield components, suggesting that selection to improve these traits should be conducted on nonsegregating plant material . Results of several research indicated that effective progress could be made through selection of morphological characters to enhance grain yield potential [27, 34–37].
The objectives of this study were to evaluate phenotypic variability, heritability, and genetic advance, to analyze relationships existing among yield and morphophysiological traits of 56 genotypes of durum wheat (Triticum durum Desf.) grown under south Mediterranean conditions, and to determine the direct and indirect contributions of the important traits with the aim to select the more desirable traits which may contribute to the improvement of durum wheat and identify the characters of utmost importance which may be used as selection criteria in a wheat breeding program.
2. Materials and Methods
2.1. Site, Plant Material, and Experimental Design
Plant material under study consisted of 56 genotypes of durum wheat (Triticum durum Desf.) (Table 1). These genotypes were evaluated in a randomized complete block design with two replications at the Field Crop Institute Agricultural Research Station (ITGC-ARS), El-Khroub, Algeria (36°17′N, 6°38′E, and 640 m above sea level), during the 2014-2015 cropping season. Plot dimensions were 4 rows, 5 m long, and 20 cm apart. Land preparation, sowing date, fertilizer application, and weed control were as recommended for the local area. The seeds were sown in early January 2015, 100 kg/ha of 46% triple superphosphate was applied at sowing and at jointing, and 130 kg/ha of 46% urea was broadcasted. Weeds were controlled chemically with Cossack® OD (mesosulfuron-methyl(mesomaxx) + iodosulfuron-methyl-sodium + mefenpyr-diethyl) at 1 l/ha rate.
2.2. Notations and Measurements
The following morphological traits were measured. Number of days to heading (DHE, days) was counted as number of calendar days from the date of sowing to the time when 50% of the spikes were half way out from the flag leaf. Plant height (PHT, cm) was measured at maturity from the soil surface up to the tip of the spike, excluding awns. Aboveground biomass (BIO, g/m), spike number (SN, #/m), straw yield (STW, g/m), and grain yield (GY, g/m) were recorded from a vegetative sample harvested from one row, 1.0 m long per plot. Harvest index (HI, %) was derived as 100 times the ratio of grain yield to aboveground biomass:
Economical yield (Yeco) was deduced as grain yield plus 0.3 times straw yield, according to Annicchiarico et al. : . At heading stage, chlorophyll content and canopy temperature were read with a chlorophyll meter type Yara N-Tester (Yara GmbH & Co. KG, Dülmen, Germany) and an infrared thermometer model Fluke 62 Max+ (Fluke Corporation, Everett, WA, USA), respectively.
2.3. Data Analysis
Collected data were subjected to an analysis of variance according to the additive model in a complete block design with two replicates, as per Steel and Torrie  (Table 2) as follows: , where is the observed value of the measured trait for ith genotype and jth block, µ is the overall mean for the measured trait, is the ith genotype effect, bj is the jth block effect, and eij is the residual associated with ith genotype and jth block.
Genotypic () and phenotypic () variance components were derived from the expected mean square according to Aquaah  as follows:
The least significance difference at 5% probability (LSD5%) was derived according to Steel and Torrie  as follows:where is the tabulated value at 5% probability with (b − 1) ( − 1) df of the residual, b is the number of blocks, and is the residual mean square. Based on the estimates of phenotypic (), genotypic (), and environmental () variances, phenotypic (PCV) and genotypic coefficients of variation (GCV) were obtained according to the method outlined by Cruz et al. :where and are the phenotypic and genotypic standard deviations and is the grand mean of the trait of interest. Broad-sense heritability () was calculated using variance components according to Acquaah :where is the genotypic variance and is the environmental variance. Selection differential (), the expected response to selection (RS), absolute value expressed as % of the grand mean (%RS) were calculated according to Cruz et al.  as follows:where is the grand mean of the trait of interest, is the mean of the selected individuals (), is the selection intensity ( = 2.06) at 5% probability level, and is the phenotypic standard deviation of the trait of interest.
Trait relationship was inspected through phenotypic (rp) and genotypic (rg) correlation coefficients, stepwise regression, and path analyses. Phenotypic (rp) and genotypic (rg) correlation coefficients were calculated as ratios of covariance to root square of the product of corresponding variances, as follows:
The genotypic covariance was derived using the property of the sum of two variables, and , whose expected mean square is as follows:
This can be rewritten as follows:
Direct and indirect effects of independent variables (i) on dependent variable (j) were obtained by decomposing correlation coefficients between dependent (j) and independent variables (i) according to Singh and Chaudhary . Statistical analyses were performed with LazStats , Past , and Cropstat  software.
3. Results and Discussion
3.1. Variability, Performance, Heritability, and Expected Response to Selection
Results of the analysis of variance indicated significant genotype effect, suggesting the presence of sizeable variation among genotypes for all measured traits (Table 3). Days to heading varied from a minimum of 117.5 to 125.0 days, with a range of 7.5 days, a value statistically significant compared to LSD5%. Phenotypic and genotypic coefficients of variation were very low in magnitude, suggesting little variability for this trait within the set of studied genotypes. Low variability was reported by Oulmi et al.  and Hannachi et al.  in durum wheat and by Fellahi et al.  in bread wheat, for this trait. Earliness is controlled by various genes related to vernalization, photoperiod, and earliness per se . When grown under heat and drought terminal stresses, plant materials drastically reduced growth cycle, minimizing variability of this trait .
DHE = days to head, BIO = aboveground biomass, SN = spike number, GY = grain yield, Yeco = economical yield, PHT = plant height, STW = straw yield, HI = harvest index, CHL = chlorophyll content, and CT = canopy temperature.
Based on a single-year data, broad-sense heritability of days to heading this trait was high, suggesting minor environmental effect, since genotypic variance was almost equal to phenotypic counterpart (Table 4). To minimize the effect of terminal heat and drought stresses, selection favored early heading genotypes; at the same time, the selection material must be late enough to avoid late spring frost [5, 48]. However, due to its low genotypic coefficient of variation, there is little scope to improve this trait through selection. Undoubtedly, earliness has been the predominant trait improved for Mediterranean conditions and may not be a prospective trait for future breeding .
DHE = days to head, BIO = aboveground biomass, SN = spike number, GY = grain yield, Yeco = economical yield, PHT = plant height, STW = straw yield, HI = harvest index, CHL = chlorophyll content, and CT = canopy temperature.
Aboveground biomass, measured at maturity, varied from 81.0 to 313.5 g/m. The range of this trait was 232.4 g/m, value significantly greater than the minimum mean of 81.0 g/m, measured for the least aboveground biomass performing genotype. Both phenotypic and genotypic coefficients of variation were high, taking values of 24.11 and 19.79%, respectively.
Broad-sense heritability was just medium, being less than 70% (Table 4). Based on genotypic coefficient of variation and broad-sense heritability, aboveground biomass appeared to be amenable to improvement, within the set of studied genotypes. González-Ribot et al.  reported a very low heritability of 9% for aboveground biomass.
Spike number varied from 31.0 to 86.0, with a range value of 55.0 spikes/m. Both phenotypic and genotypic coefficients of variation were appreciably high, taking values of 23.44 and 16.74%, respectively. Broad-sense heritability was low to medium, (51.02%), suggesting a sizeable environmental effect on the expression of this trait. In their studies, Graziani et al.  and González-Ribot et al.  found a low heritability of 27% for spike number. Similarly, grain and straw yields expressed low broad-sense heritability, high phenotypic and genotypic coefficients of variation, and significant ranges.
Plant height, chlorophyll content, and canopy temperature had high broad-sense heritability and relatively low phenotypic and genotypic coefficients of variation. The same results have been reported by Graziani et al.  and Lopes et al. , except for canopy temperature which has a moderate heritability value. Harvest index and economical yield have medium broad-sense heritability and medium to high coefficients of variation (Table 4).
Expected response to selection was relatively low for the number of days to heading (3.17%) and high for the remaining traits. The observed values were over 30.0% for biological and economical yields, over 25.00% for grain yield, plant height, and straw yield, over 20.00% for spike number and harvest index, and over 15.0% for canopy temperature and chlorophyll content (Table 4).
Similar findings have been reported by Ghaed-Rahimi and Heidari  who observed 3.10% of expected response to selection for canopy temperature, 23.93% for harvest index, and 4.18% for days to heading. However, these authors reported low values of expected response to selection for biological yield (4.68%) and grain yield (6.95%) compared to what was found in the present study.
Expected genetic gain, expressed as % of trait mean, found by Salmi et al.  varied from 4.8% for days to heading to 45.9% for grain yield. High genetic advance coupled with high heritability estimates offers the most effective condition for selection. The utility of heritability therefore increases when it is used to calculate genetic advance, which indicates the degree of gain in a character obtained under a particular selection pressure .
3.2. Trait Relationship
Study of the relationships between traits indicated that number of days to heading exhibited negative and significant phenotypic correlations with aboveground biomass (−0.273), spike number (−0.266), grain yield (−0.339), and canopy temperature (0.388). The corresponding genetic correlations were high in magnitude, except the one relating days to head to canopy temperature (Table 5).
DHE = days to head, BIO = aboveground biomass, SN = spike number, GY = grain yield, Yeco = economical yield, PHT = plant height, STW = straw yield, HI = harvest index, CHL = chlorophyll content, and CT = canopy temperature.
The weakness of the phenotypic correlations is imputable to the modifying effect of the environment which reduced the genotypic variation of the number of days to heading. Despite this, the results suggested that early genotypes tended to be more performing in terms of grain yield, aboveground biomass, and spike number and avoided terminal stresses. Low canopy temperature is associated with the ability to extract water from deeper in the soil profile . This encourages selection of early plant material.
Aboveground biomass exhibited significant and positive correlation coefficients with spike number (0.709), grain yield (0.836), economical yield (0.965), straw yield (0.962), and plant height (0.272), and negative correlation coefficients with harvest index (−0.309). Aboveground biomass, grain yield, spike number, economical yield, and straw yield were highly interrelated, at both phenotypic and genetic levels. These results suggested aboveground biomass as an indirect selection criterion to improve grain yield, along with spike number and straw and economical yields. Phenotypic correlation indicated that plant height was significantly and positively correlated with economical yield (0.300), but not with straw yield. The genetic correlations indicated that this trait had high negative correlations with canopy temperature (−4.281) and with harvest index (−1.569). Thus, plant height is usually negatively related to the HI .
This suggested that dwarf genes are linked to genes controlling high harvest index and sensitivity to high temperatures. This is suggested also by the genetic correlations of straw yield and harvest index (0.554) and straw yield and canopy temperature (2.302) (Table 5). Phenotypic correlations indicated that chlorophyll content and canopy temperature had no effect on morphological traits except that early genotypes exhibited low canopy temperature than late ones. This could be explained by the selection history of the studied genotypes, as chlorophyll content and canopy temperature were not used apart or along with grain yield and morphological traits in the selection process. But the genetic correlation indicated that genes controlling high chlorophyll content tended to be linked to those controlling high temperature tolerance. Findings about relationships between morphological and yield-related traits were in accordance with results of Leilah and Al-Khateeb  who reported that wheat grain yield was positively correlated with plant height, spike number, harvest index, and biological yield.
When subjecting the measured traits to regression analysis, the full model captured 94.58% of the total variation in grain yield and indicated that only aboveground biomass and harvest index had highly significant effect on the dependent variable. With the same set of traits, stepwise regression retained aboveground biomass and harvest index which explained 94.90% of the total variation observed in grain yield (Table 6). These results corroborated with those of Fellahi et al.  who reported that stepwise regression model retained biological yield and harvest index, which explained 99.7% of grain yield variation. In the same context, Leilah and Al-Khateeb  found that stepwise regression analysis identified spike yield, harvest index, spike number, and biological yield as the most important grain yield predictors.
Path analysis results indicated that the negative and significant correlation coefficients, relating grain yield to the number of days to heading (−0.339), were made of sizeable negative indirect effects (−0.325) via other traits and an almost nil direct effect (−0.014). The high and significant correlation coefficients relating grain yield to aboveground biomass (0.836) and to spike number (0.758) were made of high direct effect for biological yield (0.918) and indirect effects for spike number (0.690). Indirect effects of biological yield (−0.082) and direct effect of spike number (0.068), on grain yield, were too small to be of interest. The significant correlation coefficient of plant with grain yield had greater indirect effects (0.27) than direct effect (0.050). Interestingly, the nonsignificant correlation coefficient relating grain yield to harvest index was made of sizeable positive direct (0.490) and negative indirect effects (−0.271). Chlorophyll and canopy temperature had no significant direct or indirect effects on grain yield (Table 6).
Khan et al.  reported significant and positive direct effect of spike number and large indirect effects of days to heading and plant height on grain yield. The number of spikes and biological yield appeared to be the most effective variables influencing grain yield, in the present study; therefore, it is reasonable to assume that wheat grain yield, under south Mediterranean conditions, could be increased by selecting genotypes with high spike number, biological yield, and harvest index. Classifying the 56 wheat genotypes on the basis of the measured traits yielded three clusters, showing gradation, from cluster XG2 to XG1 to XG3, for biological, economical, grain, and straw yields, spike number, and harvest index. Differences between clusters for canopy temperature and number of days to heading were not large. Chlorophyll content was high in cluster XG1 and low in cluster XG3 (Table 7).
Relative differences among clusters are indicated in Figure 1. Cluster XG3 carried entries having best combinations for biological, economical, grain, and straw yields, spike number, harvest index, and plant height. Therefore, selection of suitable genotypes among the 13 available within this cluster would be beneficial, if the selected genotypes show appreciable gain over currently adopted cultivars or checks. In fact, selection of the best 5 genotypes belonging to cluster XG3 brought appreciable gains, principally for biological (10.4 to 30.3%), economical (10.3 to 31.2%), grain (13.7 to 33.7%), and straw (−2.4 to 39.3%) yields, spike number (9.9 to 20.8%), and canopy temperature (−17.3 to −21.8%). No significant change in the number of days to heading (0.0 to 3.3%) was noted.
Changes in harvest index (−11.9 to 9.7%), chlorophyll content (−9.9 to 2.4%), and plant height (−3.4 to 12.6%) were variable (Figure 2). Furthermore, choosing appropriate parents, for hybridization purposes, is an important step in plant breeding program. In fact, performance of progenies depends on parental genotype. As differences between clusters could be attributed to differences in allelic forms, crosses made between genotypes belonging to different clusters are expected to generate variability within which selection to improve the targeted traits would be more efficient.
Altogether, results of the present study indicated that the existing variability varied among traits, being low for number of days to heading, plant height, chlorophyll content, and canopy temperature and high for biological, grain, economical, and straw yields and harvest index. Heritability was low for grain and straw yields, medium for biological and economical yields, spike number, and harvest index, and high for days to heading, spike number, plant height, chlorophyll content, and canopy temperature. Expected response to selection was relatively low for the number of days to heading and high for the remaining traits. These results corroborate findings reported by Salmi et al.  and Ghaed-Rahimi and Heidari .
The results of correlation coefficients indicated that early genotypes performed best in terms of grain yield, aboveground biomass, and spike number and avoided terminal heat stress, encouraging selection of early plant material. Correlation coefficient results pointed to aboveground biomass as an indirect selection criterion to improve grain yield, along with spike number, straw yield, economical yield, and harvest index. These findings were in accordance with results of Fellahi et al. , Salmi et al. , Hannachi et al. , and Leilah and Al-Khateeb . Path analysis indicated that days to heading and spike number affected grain yield mainly through indirect effects, while aboveground biomass had high direct effect. Harvest index had both significant positive direct and negative indirect effects. Three contrasted clusters were extracted from the data set.
Crosses between genotypes belonging to different clusters to generate variability within which selection to improve the targeted traits would be more efficient are proposed. This increases possibilities to improve both yield potential and stress tolerance. Selection should be done, preferentially for spike number, biological yield, harvest index, and canopy temperature to accumulate favorable alleles in the selected entries. Selection of best genotypes, for direct uses as cultivars, from clusters carrying best combinations of yield-related traits is feasible.
Sufficient variability was noted for the measured traits in the data set obtained from the 56 assessed genotypes. Heritability and expected genetic gain varied among the measured traits. Relationship analysis designated aboveground biomass, harvest index, and spike number as the most grain yield-influencing traits. High genetic advance coupled with high heritability estimates offers the most effective condition for selection. The utility of heritability therefore increases when it is used to calculate genetic advance, which indicates the degree of gain in a character obtained under a particular selection pressure. Selection for earliness is encouraging since early genotypes exhibited above-average grain and biological yields and spike number and most importantly avoided terminal heat stress. Selection for direct use from cluster carrying best combination of yield-related traits and crosses among genotypes belonging to contrasted clusters were suggested to generate more variability. Selection should be done, preferentially for spike number, biological yield, harvest index, and canopy temperature to accumulate favorable alleles in the selected entries for future uses. The results suggest that genotypes with high expression of biomass (source), good harvest index expression, and other yield components can be used as parents in durum wheat breeding program to boost the genetic gains.
This work is part of the Ph.D. thesis of Ali Mansouri.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this article.
The authors would like to express special thanks to the personnel of INRAA-ITGC-ARS of El-Khroub, particularly durum wheat breeding team for kindly providing seeds and facilities and help in conducting the experiment.
- Food and Agricultural Organization of the United Nations, Statistics Division, FAOSTAT, 2016, http://faostat3.fao.org/home/e.
- M. Graziani, M. Maccaferri, C. Royo, F. Salvatorelli, and R. Tuberosa, “QTL dissection of yield components and morpho-physiological traits in a durum wheat elite population tested in contrasting thermo-pluviometric conditions,” Crop and Pasture Science, vol. 65, no. 1, pp. 80–95, 2014.
- J. Subira, F. Álvaro, L. F. G. Del Moral, and C. Royo, “Breeding effects on the cultivar x environment interaction of durum wheat yield,” European Journal of Agronomy, vol. 68, pp. 78–88, 2015.
- J. L. Araus, J. Bort, P. Steduto, D. Villegas, and C. Royo, “Breeding cereals for Mediterranean conditions: ecophysiological clues for biotechnology application,” Annals of Applied Biology, vol. 142, no. 2, pp. 129–141, 2003.
- G.A. Slafer, J. L. Araus, C. Royo, and L. F. G. Del Moral, “Promising eco-physiological traits for genetic improvement of cereal yields in Mediterranean environments,” Annals of Applied Biology, vol. 146, no. 1, pp. 61–70, 2005.
- P. Annicchiarico, Genotype x Environment Interactions: Challenges and Opportunities for Plant Breeding and Cultivar Recommendations, Food and Agriculture Organization, Rome, Italy, 2002.
- E. Acevedo and P. Silva, “Trigo candeal: calidad, mercado y zonas de cultivo,” in Serie Ciencias Agronómicas, Universidad de Chile, Facultad de Ciencias Agronómicas, Región Metropolitana, Chile, 2007.
- R. Motzo, F. Giunta, and G. Pruneddu, “The response of rate and duration of grain filling to long-term selection for yield in Italian durum wheats,” Crop and Pasture Science, vol. 61, no. 2, pp. 162–169, 2010.
- A. Blum, Plant Breeding for Water-Limited Environments, Springer Science & Business Media, New York, NY, USA, 2011.
- M. Lopes, M. Reynolds, M. Jalal-Kamali et al., “The yield correlations of selectable physiological traits in a population of advanced spring wheat lines grown in warm and drought environments,” Field Crops Research, vol. 128, pp. 129–136, 2012.
- J. Crossa, G. de los Campos, M. Maccaferri, R. Tuberosa, J. Burgueño, and P. Pérez-Rodríguez, “Extending the marker × environment interaction model for genomic-enabled prediction and genome-wide association analysis in durum wheat,” Crop Science, vol. 56, no. 5, pp. 2193–2209, 2016.
- R. A. Fisher, “Wheat physiology: a review of recent developments,” Crop and Pasture Science, vol. 62, no. 2, pp. 95–114, 2011.
- C. Royo, R. Nazco, and D. Villegas, “The climate of the zone of origin of Mediterranean durum wheat (Triticum durum Desf.) landraces affects their agronomic performance,” Genetic Resources and Crop Evolution, vol. 61, no. 7, pp. 1345–1358, 2014.
- L. Mwadzingeni, H. Shimelis, S. Tesfay, and T. J. Tsilo, “Screening of bread wheat genotypes for drought tolerance using phenotypic and proline analyses,” Frontiers in Plant Science, vol. 7, p. 1276, 2016.
- D. Dodig, M. Zoriæ, V. Kandiæ, D. Peroviæ, and G. Šurlan−Momiroviæ, “Comparison of responses to drought stress of 100 wheat accessions and landraces to identify opportunities for improving wheat drought resistance,” Plant Breeding, vol. 131, no. 3, pp. 369–379, 2012.
- N. N. Hamssi and A. Najaphy, “Comparison of photosynthetic components of wheat genotypes under rain-fed and irrigated conditions,” Photochemistry and Photobiology, vol. 88, no. 1, pp. 76–80, 2012.
- G. J. Rebetzke, A. Rattey, G. D. Farquhar, R. A. Richards, and A. G. Condon, “Genomic regions for canopy temperature and their genetic association with stomatal conductance and grain yield in wheat,” Functional Plant Biology, vol. 40, no. 1, pp. 14–33, 2013.
- M. Tattaris, M. P. Reynolds, and S. C. Chapman, “A direct comparison of remote sensing approaches for high-throughput phenotyping in plant breeding,” Frontiers in Plant Science, vol. 7, p. 1131, 2016.
- J. J. Olivares-Villegas, M. P. Reynolds, and G. K. McDonald, “Drought adaptive attributes in the Seri/Babax hexaploid wheat population,” Functional Plant Biology, vol. 34, no. 3, pp. 189–203, 2007.
- C. Royo, D. Villegas, L. F. G. Del Moral et al., “Comparative performance of carbon isotope discrimination and canopy temperature depression as predictors of genotype differences in durum wheat yield in Spain,” Australian Journal of Agricultural Research, vol. 53, no. 5, pp. 561–569, 2002.
- F. Giunta, R. Motzo, and G. Pruneddu, “Has long-term selection for yield in durum wheat also induced changes in leaf and canopy traits?” Field Crops Research, vol. 106, no. 1, pp. 68–76, 2008.
- G. A. Slafer, V. O. Sadras, and S. Roxana, Is Compensation between Yield Components in Wheat a Feedback Process of Responses to Availability of Resources? 2008, http://www.intlcss.org/files/icss/congress-proceedings/2008-papers/cs3-s1/cs3-s1-o3-gustavo-slafer.pdf.
- G. A. Slafer, R. Savin, and V. O. Sadras, “Coarse and fine regulation of wheat yield components in response to genotype and environment,” Field Crops Research, vol. 157, pp. 71–83, 2014.
- V. O. Sadras, “Evolutionary aspects of the trade-off between seed size and number in crops,” Field Crops Research, vol. 100, pp. 125–138, 2007.
- G. González-Ribot, M. Opazo, P. Silva, and E. Acevedo, “Traits explaining durum wheat (Triticum turgidum L. spp. durum) yield in dry Chilean Mediterranean environments,” Frontiers in Plant Science, vol. 8, p. 1781, 2017.
- T. Mohsin, N. Khan, and F.N. Naqvi, “Heritability, phenotypic correlation and path coefficient studies for some agronomic characters in synthetic elite lines of wheat,” Journal of Food, Agriculture and Environment, vol. 7, no. 3-4, pp. 278–282, 2009.
- Z. Fellahi, A. Hannachi, H. Bouzerzour, and A. Boutekrabt, “Line × tester mating design analysis for grain yield and yield related traits in bread wheat (Triticum aestivum L.),” International Journal of Agronomy, vol. 2013, Article ID 201851, 9 pages, 2013.
- M. Kandel, A. Bastola, P. Sapkota, O. Chaudhary, P. Dhakal, and P. Chalise, “Association and path coefficient analysis of grain yield and its attributing traits in different genotypes of wheat (Triticum aestivum L.),” International Journal of Applied Sciences and Biotechnology, vol. 5, no. 4, pp. 449–453, 2017.
- Z. Fellahi, A. Hannachi, H. Bouzerzour, and A. Benbelkacem, “Inheritance pattern of metric characters affecting grain yield in two bread wheat (Triticum aestivum L.) crosses under rainfed conditions,” Jordan Journal of Biological Sciences, vol. 8, no. 3, pp. 175–181, 2015.
- C. Royo, E. M. Elias, and F. A. Manthey, “Durum wheat breeding,” in Handbook of Plant Breeding: Cereals, M. J. Carena, Ed., pp. 199–226, Springer, New York, NY, USA, 2009.
- M. Salmi, L. Haddad, A. Oulmi, A. Benmahammed, and A. Benbelkacem, “Variabilité phénotypique et sélection des caractères agronomiques du blé dur (Triticum durum Desf.) sous conditions semi-arides,” European Scientific Journal, vol. 11, no. 2, pp. 99–111, 2015.
- V. K. Arya, J. Singh, L. Kumar et al., “Character association and path coefficient analysis in wheat (Triticum aestivum L.),” Indian Journal of Agricultural Research, vol. 51, no. 3, pp. 245–251, 2017.
- A. Hannachi, Z. Fellahi, A. Rabti, A. Guendouz, and H. Bouzerzour, “Combining ability and gene action estimates for some yield attributes in durum wheat (Triticum turgidum L. var. durum),” Journal of Fundamental and Applied Sciences, vol. 9, no. 3, pp. 1519–1534, 2017.
- A. Hannachi, Z. Fellahi, H. Bouzerzour, and A. Boutekrabt, “Diallel-cross analysis of grain yield and stress tolerance-related traits under semi-arid conditions in durum wheat (Triticum durum Desf.),” Electronic Journal of Plant Breeding, vol. 4, pp. 1027–1033, 2013.
- M. Moghaddam, B. Ehdaie, and J. G. Waines, “Genetic variation for and interrelationships among agronomic traits in landraces of bread wheat from southwestern Iran,” Journal of Genetics and Breeding, vol. 52, no. 1, pp. 73–81, 1998.
- V. Mollasadeghi, R. Shahryari, A. A. Imani, and M. Khayatnezhad, “Factor analysis of wheat quantitative traits on yield under terminal drought,” American-Eurasian Journal of Agriculture and Environmental Science, vol. 10, no. 2, pp. 157–159, 2011.
- M. Zakizadeh, M. Esmaeilzadeh, and D. Kahrizi, “Study on genetic variation and relationship between plant characteristics and yield in long spike bread wheat (Triticum aestivum L.) genotypes using multivariate analysis,” Iranian Journal of Crop Sciences, vol. 12, no. 2, pp. 18–30, 2010.
- P. Annicchiarico, F. Bellah, and T. Chiari, “Repeatable genotype x location interaction and its exploitation by conventional and GIS-based cultivar recommendation for durum wheat in Algeria,” European Journal of Agronomy, vol. 24, no. 1, pp. 70–81, 2006.
- R. G. D. Steel and J. H. Torrie, Principles and Procedures of Statistics, McGraw-Hill Books, New York, NY, USA, 1982.
- G. Acquaah, Principles of Plant Genetics and Breeding, John Wiley and Sons, Hoboken, NJ, USA, 2009.
- C. D. Cruz, A. J. Regazi, and P. C. S. Carneiro, Modelos Biométricos Aplicados ao Melhoramento Genético, UFV, Viçosa, Brazil, 4th edition, 2012.
- R. K. Singh and B. D. Chaudhary, Biometrical Methods in Quantitative Genetic Analysis, Kalyani Publishers, New Delhi, India, 1985.
- LazStats, Free Statistics Programs and Materials, W. G. Miller, 2013.
- O. Hammer, D. A. T. Harper, and P. D. Ryan, “PAST: paleontological statistics software package for education and data analysis,” Paleontologia Electronica, vol. 4, pp. 1–9, 2001.
- Cropstat, Cropstat for Windows 2007.7.2.3, International Rice Research Institute, Manila, Philippines, 2007.
- A. Oulmi, A. Benmahammed, Z. Laala, A. Adjabi, and H. Bouzerzour, “Phenotypic variability and relations between the morpho-physiological traits of three F5 populations of durum wheat (Triticum durum Desf.) evaluated under semi-arid conditions,” Advances in Environmental Biology, vol. 8, pp. 436–443, 2015.
- Z. Fellahi, A. Hannachi, H. Bouzerzour, and A. Benbelkacem, “Genetic control of bread wheat (Triticum aestivum L.) traits,” Songklanakarin Journal of Science and Technology, vol. 38, no. 1, pp. 91–97, 2016.
- A. Mekhlouf, F. Dehbi, H. Bouzerzour, A. Hannachi, A. Benmahammed, and A. Adjabi, “Relationships between cold tolerance, grain yield performance and stability of durum wheat (Triticum durum Desf.) genotypes grown at high elevation area of eastern Algeria,” Asian Journal of Plant Science, vol. 5, pp. 700–708, 2006.
- L. Ghaed-Rahimi and B. Heidari, “Genetic advance prediction and multivariate analysis for antioxidants and agronomic traits in wheat,” Annual Research and Review in Biology, vol. 4, no. 14, pp. 2427–2449, 2014.
- C. Royo, F. Alvaro, V. Martos et al., “Genetic changes in durum wheat yield components and associated traits in Italian and Spanish varieties during the 20th century,” Euphytica, vol. 155, no. 1-2, pp. 259–270, 2007.
- A. A. Leilah and S. A. Al-Khateeb, “Statistical analysis of wheat yield under drought conditions,” Journal of Arid Environments, vol. 61, no. 3, pp. 483–496, 2005.
- A. A. Khan, M. A. Alam, M. K. Alam, M. J. Alam, and Z. I. Sarker, “Correlation and path analysis of durum wheat (Triticum turgidum L. var. durum),” Bangladesh Journal of Agriculture Research, vol. 38, no. 3, pp. 515–521, 2013.
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