Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Figure 1

In vitro regeneration of plantlets from leaf disc explants of passion fruit variety KPF 4 cultured on MS medium and acclimatization. (a) and (b) Initiation of shoots from leaf disc explants cultured on MS + 2 mg·L⁻¹ BAP on the 3^rd and 4^th weeks after culture, respectively; (c) and (d) further development of shoots on MS + 0.1 mg·L⁻¹ BAP on the 2^nd and 4^th weeks after culture, respectively; (e) and (f) rooted plants on MS + 0.1 mg·L⁻¹ NAA on the 1^st and 2^nd weeks after culture, respectively; (g) acclimatization stage (2-week- old plant in a plastic pot); (h) one-month-old acclimatized plant in a plastic pot.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)