In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia

Table 2

Interaction effects of PGRs supplements and genotypes on the mean shoot number.


Genotype	Treatments
Genotype	T1	T2	T3	T4	T5

Aberghele	4.00 ± 0.00^l–z	8.33 ± 1.53^d–g	10.33 ± 1.53^a–d	6.00 ± 1.73^h–l	11.67 ± 1.53^ab
ACC44	2.33 ± 1.15^x–B	5.33 ± 1.15^j–q	5.33 ± 1.15^j–q	9.67 ± 0.58^b–f	8.00 ± 0.00^e–h
ADI	3.00 ± 1.00^q–B	4.67 ± 1.15^j–x	5.00 ± 1.00^j–v	6.33 ± 2.08^g–k	4.67 ± 2.08^j–x
Baha Necho	1.33 ± 0.58^AB	4.00 ± 0.00^l–z	4.33 ± 1.53^k–y	6.67 ± 1.15^g–j	4.00 ± 1.00^l–z
Baha Zeyit	3.00 ± 1.73^q–B	10.67 ± 0.58^a–c	6.67 ± 0.58^g–j	9.67 ± 0.58^b–f	8.00 ± 0.00^e–h
Borkena	1.33 ± 0.58^AB	3.00 ± 0.00^q–B	3.67 ± 0.58^l–z	3.33 ± 1.53^o–A	6.00 ± 1.00^h–m
Bounji	6.67 ± 1.15^v–B	5.33 ± 1.15^j–q	7.67 ± 0.58^f–i	4.00 ± 0.00^l–z	6.67 ± 1.15^g–j
Gondar 1	1.00 ± 0.00^B	3.00 ± 1.00^z–B	2.67 ± 0.58^v–B	4.00 ± 0.00^l–z	3.33 ± 0.58^o–A
Gumero	3.67 ± 0.58^l–z	3.67 ± 0.58^l–z	3.67 ± 0.58^l–z	3.00 ± 1.00^q–B	4.00 ± 0.00^l–z
Hirhir	3.06 ± 1.00^q–B	6.00 ± 1.73^h–l	6.67 ± 1.53^g–j	10.67 ± 1.15^a–c	12.00 ± 0.00^a
Humera 1	4.67 ± 1.15^j–x	5.67 ± 1.53^i–o	11.33 ± 1.53^a–c	10.00 ± 1.73^a–e	10.33 ± 1.53^a–d
Setit 1	4.00 ± 0.00^l–z	5.33 ± 1.15^j–q	5.33 ± 1.15^j–q	4.67 ± 1.15^j–x	6.00 ± 2.00^h–m
Setit 2	4.00 ± 0.00^l–z	4.00 ± 0.58^i–o	4.00 ± 0.00^l–z	4.67 ± 1.15^j–x	4.00 ± 0.00^l–z
Zeri Tesfay	5.67 ± 1.53^i–o	8.00 ± 2.00^e–h	10.00 ± 2.00^a–e	9.33 ± 1.15^c–f	9.67 ± 2.08^b–f

Treatments: T1: 0.00 mg L⁻¹ BAP ± 0.00 mg·L⁻¹ NAA; T2: 0.25 mg·L⁻¹ BAP ± 1.00 mg·L⁻¹ NAA; T3: 0.50 mg·L⁻¹ BAP ± 1.00 mg·L⁻¹ NAA; T4: 0.75 mg·L⁻¹ BAP ± 1.00 mg·L⁻¹ NAA; T5: 1.00 mg·L⁻¹ BAP ± 1.00 mg·L⁻¹ NAA (CV%: 20.1; SEM: 0.662). Means followed by a different letter indicate significant differences at P ≤ 0.01.

International Journal of Agronomy

In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia

Table 2