Isolation and Characterization of Chickpea (Cicer arietinum L.) Nodulating Rhizobia Collected from South Wollo Zone, Ethiopia

Table 1

Performance of selected rhizobia isolates on potted sterilized sand under greenhouse conditions.

Isolates	Collection sites (Woreda)	SL/P	NN/P	NDW/p	SDW/p	SE (%)	Status
WUCR-1	Tehuledere	30.7 ± 2.3^b-g	56.33 ± 12.79^ab	0.173 ± 0.009^bc	0.777 ± 0.020^a−−c	152.1	HE
WUCR-2	Tehuledere	32.7 ± 1.2^c−h	26.00 ± 2.08^f−m	0.110 ± 0.012^b	0.740 ± 0.029^a−d	128.9	HE
WUCR-3	Tehuledere	26.2 ± 1.9^a−d	38.00 ± 6.03^c−j	0.073 ± 0.003^b	0.553 ± 0.075^c−l	98.2	HE
WUCR-5	Tehuledere	30.2 ± 1.7^b−g	46.33 ± 1.45^b−e	0.100 ± 0.006^b	0.567 ± 0.079^b−k	101.6	HE
WUCR-6	Tehuledere	29.8 ± 0.6^b−g	39.33 ± 2.33^b−j	0.133 ± 0.009^bc	0.663 ± 0.073^a−e	113.6	HE
WUCR-8	Tehuledere	32.2 ± 2.6^b−h	51.33 ± 12.12^b−d	0.147 ± 0.015^bc	0.787 ± 0.099^a−c	137.8	HE
WUCR-9	Tehuledere	29.0 ± 0.6^b−g	51.00 ± 0.58^b−d	0.110 ± 0.015^b	0.563 ± 0.009^b−k	98.8	HE
WUCR-11	Tehuledere	30.7 ± 1.8^b−g	36.67 ± 2.85^d−l	0.097 ± 0.022^b	0.620 ± 0.105^a−h	111.3	HE
WUCR-12	Tehuledere	29.5 ± 1.6^b−g	19.00 ± 5.57^l−m	0.087 ± 0.002^b	0.420 ± 0.025^f−m	74.2	E
WUCR-14	Tehuledere	27.7 ± 1.5^b−g	23.67 ± 1.67^i−m	0.070 ± 0.006^b	0.360 ± 0.023^i−m	76.3	E
WUCR-15	Tehuledere	32.5 ± 0.8^c−h	40.67 ± 3.84^b−i	0.130 ± 0.010^bc	0.793 ± 0.043^a−b	137.6	HE
WUCR-16	Tehuledere	25.8 ± 2.1^a−c	22.33 ± 2.73^j−m	0.070 ± 0.010^b	0.573 ± 0.052^a−j	101	HE
WUCR-17	Tehuledere	32.8 ± 2.6^d−h	68.67 ± 0.88^a	0.123 ± 0.015^b	0.787 ± 0.146^a−c	140.6	HE
WUCR-18	Tehuledere	29.3 ± 1.7^b−g	31.33 ± 4.37^e−m	0.093 ± 0.012^b	0.333 ± 0.028^bc	59	E
WUCR-22	Kutaber	33.2 ± 1.8^e−h	37.00 ± 5.51^d−k	0.117 ± 0.012^b	0.703 ± 0.105^a−d	120.2	HE
WUCR-23	Kutaber	34.2 ± 2.9^gh	33.67 ± 6.94^d−l	0.107 ± 0.007^b	0.680 ± 0.065^a−d	116.9	HE
WUCR-25	Kutaber	27.0 ± 1.0^b−f	19.67 ± 3.18^k−m	0.073 ± 0.015^b	0.340 ± 0.040^k−m	58.3	E
WUCR-26	Kutaber	26.3 ± 2.0^a−e	31.67 ± 7.69^e−m	0.087 ± 0.012^b	0.607 ± 0.092^a−h	108.7	HE
WUCR-28	Kutaber	31.7 ± 1.1^b−h	40.67 ± 2.96^b−i	0.097 ± 0.007^bc	0.727 ± 0.048^a−d	126.9	HE
WUCR-29	Kutaber	30.7 ± 2.0^b−g	36.67 ± 2.73^d−l	0.113 ± 0.019^b	0.533 ± 0.033^d−l	94.3	HE
WUCR-30	Kutaber	25.8 ± 1.3^a−c	37.00 ± 1.16^d−k	0.087 ± 0.003^b	0.347 ± 0.033^j−m	59.8	E
WUCR-31	Kutaber	28.7 ± 1.5^b−g	24.33 ± 4.41^g−m	0.089 ± 0.005^bc	0.360 ± 0.038^i−m	63.4	E
WUCR-33	Kutaber	30.3 ± 0.3^b−g	36.33 ± 6.69^d−l	0.100 ± 0.015^b	0.770 ± 0.084^a−d	133.1	HE
WUCR-34	Kutaber	29.3 ± 0.7^b−g	41.67 ± 4.91^b−h	0.107 ± 0.020^b	0.777 ± 0.044^a−c	135.6	HE
WUCR-35	Kutaber	31.7 ± 1.2^b−h	40.33 ± 3.48^b−i	0.133 ± 0.022^bc	0.710 ± 0.053^a−d	122.9	HE
WUCR-37	Kutaber	25.3 ± 0.7^ab	14.67 ± 4.84^mn	0.087 ± 0.003^b	0.277 ± 0.024^c	47.6	LE
WUCR-39	Kutaber	28.3 ± 3.3^b−g	43.67 ± 7.31^b−f	0.120 ± 0.029^b	0.590 ± 0.150^a−i	107.4	HE
WUCR-46	Kutaber	28.3 ± 1.2^b−g	38.33 ± 2.60^c−j	0.103 ± 0.003^b	0.407 ± 0.015^g−m	70.9	E
WUCR-49	Kutaber	32.5 ± 1.3^c−h	37.67 ± 3.28^c−j	0.090 ± 0.012^b	0.727 ± 0.073^a−d	128.1	HE
WUCR-51	Kutaber	30.7 ± 0.7^b−g	26.00 ± 3.61^f−m	0.090 ± 0.000^b	0.427 ± 0.015^f−m	74.3	E
WUCR-55	Kutaber	29.0 ± 0.6^b−g	40.67 ± 2.60^b−i	0.110 ± 0.006^b	0.670 ± 0.068^a−e	117.8	HE
WUCR-56	Kutaber	37.5 ± 9.0^h	28.33 ± 8.99^f−m	0.087 ± 0.033^b	0.400 ± 0.038^h−m	69.4	E
WUCR-57	Kutaber	33.0 ± 0.0^d−h	28.00 ± 2.00^f−m	0.610 ± 0.255^a	0.713 ± 0.043^a−d	123.6	HE
WUCR-58	Kutaber	29.0 ± 0.6^b−g	38.67 ± 2.85^c−j	0.100 ± 0.006^b	0.633 ± 0.023^a−g	111	HE
WUCR-59	Kutaber	33.5 ± 0.5^f−h	34.33 ± 4.41^d−l	0.130 ± 0.006^bc	0.713 ± 0.049^a−d	125.5	HE
WUCR-61	Kutaber	27.8 ± 0.9^b−g	24.00 ± 1.00^h−m	0.077 ± 0.009^b	0.443 ± 0.055^e−m	78.1	E
WUCR-62	Kutaber	30.0 ± 0.0^b−g	42.00 ± 5.00^b−g	0.100 ± 0.025^b	0.593 ± 0.032^a−h	103.2	HE
WUCR-65	Kutaber	29.3 ± 0.7^b−g	30.00 ± 8.08^e−m	0.120 ± 0.006^b	0.557 ± 0.062^b−l	99.3	HE
WUCR-66	Kutaber	32.8 ± 0.6^d−h	54.67 ± 3.71^a−c	0.127 ± 0.013^b	0.810 ± 0.070^a	141.7	HE
WUCR-74	Kutaber	31.7 ± 1.2^b−h	32.00 ± 9.02^e−m	0.107 ± 0.015^b	0.700 ± 0.103^a−d	121.6	HE
WUCR-76	Kutaber	32.0 ± 1.2^b−h	34.33 ± 2.03^d−l	0.133 ± 0.022^bc	0.560 ± 0.071^b−l	99.8	HE
WUCR-82	Kutaber	29.0 ± 1.0^b−g	34.00 ± 4.36^d−l	0.103 ± 0.009^b	0.663 ± 0.118^a−e	112.2	HE
WUCR-89	Kutaber	31.7 ± 2.2^b−h	35.00 ± 6.66^d−l	0.113 ± 0.024^b	0.580 ± 0.035^a−i	101.3	HE
WUCR-101	Kutaber	27.7 ± 1.3^b−g	22.33 ± 2.40^j−m	0.291 ± 0.210^b	0.277 ± 0.020^c	48.7	LE
WUCR-105	Kutaber	31.3 ± 1.2^b−h	31.67 ± 5.70^e−m	0.113 ± 0.015^b	0.680 ± 0.132^a−d	115.9	HE
WUCR-106	Kutaber	28.5 ± 1.0^b−g	36.67 ± 1.76^d−l	0.103 ± 0.009^b	0.643 ± 0.033^a−f	112.7	HE
− Cont.	—	20.5 ± 0.8^a	0.00 ± 0.00^n	0.000 ± 0.000^c	0.240 ± 0.040^c	40.9	—
+ Cont.	—	27.3 ± 0.3^b−g	0.00 ± 0.00^n	0.000 ± 0.000^c	0.583 ± 0.058^a−i	100	—
Total	—	29.9 ± 0.3	34.10 ± 1.19	0.115 ± 0.009	0.583 ± 0.015	102.5	—

SL/p = shoot length per plant, NN/p = module number per plant, NDW/p = nodule dry weight per plant, SDW/p = shoot dry weight per plant, RE = relative effectiveness, E = effective, LE = lowly effective, HE = highly effective, HE >80%, E 50–80%, LE 35–50%, IE <35%, the same letters are not significantly different at level, and all the data are the means of triplicates. The unit for measuring SL/p is centimeter and the other NDW/p and SDW/p are in gram.