International Journal of Agronomy

Research Article

Physiological and Molecular Response of Wheat Cultivars to Titanium Dioxide or Zinc Oxide Nanoparticles under Water Stress Conditions

Table 3

SRAP-PCR analysis of two wheat cultivars (Gimeza 12 & Sids 13) treated with nano TiO2 or ZnO (10mg/l) subjected to different levels of water irrigation requirments at 75 days from sowing.
(a) SRAP primers used in this study
Primer’s nameSequence
ForwardReverse
SRAP1TGAGTCCAAACCGGATAGACTGCGTACGAATTAAT
SRAP2TGAGTCCAAACCGGAGCGACTGCGTACGAATTTGC
SRAP3TGAGTCCAAACCGGAATGACTGCGTACGAATTGAC
SRAP4TGAGTCCAAACCGGACCGACTGCGTACGAATTTGA
SRAP5TGAGTCCAAACCGGAAGGACTGCGTACGAATTAAC
SRAP6TGAGTCCAAACCGGTCAGACTGCGTACGAATTACG
(b) SRAP-PCR analysis of two wheat cultivars treated and untreated with nanoparticles
Primer code no.Size range of the scorable loci (bp)Total lociNo. of monomorphic lociNo. of polymorphic loci% polymorphismUnique lociMolecular size of markers (bp)
SRAP-190–85073457.1400
SRAP-2330–49532133.331233-
SRAP-3250–85065116.6600
SRAP-4133–36132133.3300
SRAP-5160–50052360.0000
SRAP-6125–51232133.3300
Total90–85027161140.74%13.70%