SCoT, ISSR, and SDS-PAGE Investigation of Genetic Diversity in Several Egyptian Wheat Genotypes under Normal and Drought Conditions

Table 3

Primer sequences, P%, PIC, MI, and Rp in SCoT as well as ISSR primers produced in the ten wheat genotypes.

Marker	Primer	Primer sequences 5ʹ-3ʹ	TAF	PF	MF	UF	P (%)	PIC	MI	Rp	FS (bp)
											Small	Large
SCoT	SCoT1	ACGACATGGCGACCACGC3	5.0	4.0	1.0	1.0	80	0.55	1.76	4.0	350	1500
	SCoT2	ACCATGGCTACCACCGGC	9.0	7.0	2.0	1.0	77.8	0.57	3.1	6.4	510	1520
	SCoT3	ACGACATGGCGACCCACA	6.0	6.0	0.0	0.0	100	0.87	5.22	4.0	205	800
	SCoT4	ACCATGGCTACCACCGCA	6.0	5.0	1.0	0.0	83.3	0.21	0.87	8.6	575	1500
	SCoT5	CAATGGCTACCACTAGCG	10.0	10.0	0.0	1.0	100	0.80	8.0	8.2	200	1350
	SCoT10	ACAATGGCTACCACCAGC	14.0	14.0	0.0	0.0	100	0.75	10.5	13.0	240	1800
	SCoT12	CAACAATGGCTACCACCG	11.0	11.0	0.0	1.0	100	0.54	5.9	13.8	245	1215
Ave.			8.71	8.14	0.57	0.57	91.58	0.61	5.05	8.28
ISSR	ISSR1	AGAGAGAGAGAGAGAGYC	7.0	4.0	3.0	1.0	57.1	0.34	0.77	4.4	120	780
	ISSR2	AGAGAGAGAGAGAGAGYG	7.0	6.0	1.0	3.0	85.7	.074	3.8	3.2	100	1000
	ISSR3	ACACACACACACACACYT	10.0	9.0	1.0	5.0	90	0.7	5.67	6.4	200	1300
	ISSR4	ACACACACACACACACYG	9.0	8.0	1.0	2.0	88.9	0.72	5.76	5.6	290	930
	ISSR6	CGCGATAGATAGATAGATA	8.0	6.0	2.0	1.0	75	0.60	2.7	5.0	330	1800
	ISSR11	ACACACACACACACACYA	6.0	4.0	2.0	3.0	66.7	0.58	1.5	2.0	320	1000
	ISSR12	ACACACACACACACACYC	4.0	3.0	1.0	1.0	75	0.38	0.85	3.6	310	495
Ave.			7.28	5.71	1.57	2.28	76.91	0.48	3.0	4.31

TAF, total amplified fragments; PF, polymorphic fragments; MF, monomorphic fragments; UF, unique fragments; P%, percentage of polymorphism; PIC, polymorphism information content; MI, marker index; Rp, resolving power; and FS, fragment size.