Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions
Table 2
Total chlorophyll (Chl T), membrane stability index (MSI), and relative water content (RWC) in H. pusillus calli cultures treated with melatonin and PEG levels after 28 days.
Treatments
Chl T (mg g−1 FW)
MSI
RWC (%)
PEG 0% + melatonin 0 mg l−1
2.47 ± 0.121ef
75.49 ± 2.030fg
82.88 ± 0.697f
PEG 0% + melatonin 0.5 mg l−1
2.62 ± 0.025gh
73.61 ± 1.581f
82.06 ± 1.320f
PEG 0% + melatonin 1.0 mg l−1
2.85 ± 0.076i
80.23 ± 2.096h
83.93 ± 2.165f
PEG 0% + melatonin 1.5 mg l−1
2.72 ± 0.123h
85.21 ± 1.616i
87.03 ± 1.243g
PEG 0% + melatonin 2.0 mg l−1
2.52 ± 0.090fg
86.48 ± 2.761i
87.08 ± 0.656g
PEG 4% + melatonin 0 mg l−1
0.99 ± 0.084a
45.48 ± 2.336a
57.41 ± 1.234a
PEG 4% + melatonin 0.5 mg l−1
1.43 ± 0.139b
54.19 ± 2.237b
66.14 ± 1.279b
PEG 4% + melatonin 1.0 mg l−1
1.95 ± 0.100c
57.22 ± 1.257c
67.73 ± 2.041c
PEG 4% + melatonin 1.5 mg l−1
2.14 ± 0.031d
63.11 ± 1.926d
72.07 ± 1.206d
PEG 4% + melatonin 2.0 mg l−1
2.15 ± 0.174d
66.77 ± 1.424e
75.28 ± 1.252e
Data show the mean values ± standard errors of triplicates. Different alphabets indicate significant statistical differences between the mean values based on Duncan’s test at .