Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions

Table 2

Total chlorophyll (Chl T), membrane stability index (MSI), and relative water content (RWC) in H. pusillus calli cultures treated with melatonin and PEG levels after 28 days.

Treatments	Chl T (mg g−1 FW)	MSI	RWC (%)
PEG 0% + melatonin 0 mg l^−1	2.47 ± 0.121^ef	75.49 ± 2.030^fg	82.88 ± 0.697^f
PEG 0% + melatonin 0.5 mg l^−1	2.62 ± 0.025^gh	73.61 ± 1.581^f	82.06 ± 1.320^f
PEG 0% + melatonin 1.0 mg l^−1	2.85 ± 0.076^i	80.23 ± 2.096^h	83.93 ± 2.165^f
PEG 0% + melatonin 1.5 mg l^−1	2.72 ± 0.123^h	85.21 ± 1.616^i	87.03 ± 1.243^g
PEG 0% + melatonin 2.0 mg l^−1	2.52 ± 0.090^fg	86.48 ± 2.761^i	87.08 ± 0.656^g
PEG 4% + melatonin 0 mg l^−1	0.99 ± 0.084^a	45.48 ± 2.336^a	57.41 ± 1.234^a
PEG 4% + melatonin 0.5 mg l^−1	1.43 ± 0.139^b	54.19 ± 2.237^b	66.14 ± 1.279^b
PEG 4% + melatonin 1.0 mg l^−1	1.95 ± 0.100^c	57.22 ± 1.257^c	67.73 ± 2.041^c
PEG 4% + melatonin 1.5 mg l^−1	2.14 ± 0.031^d	63.11 ± 1.926^d	72.07 ± 1.206^d
PEG 4% + melatonin 2.0 mg l^−1	2.15 ± 0.174^d	66.77 ± 1.424^e	75.28 ± 1.252^e

Data show the mean values ± standard errors of triplicates. Different alphabets indicate significant statistical differences between the mean values based on Duncan’s test at .