Effect of Dissolved Oxygen and Chemical Scarification on Andrographis paniculata Seed Germination in Macrobubble Conditions

Table 2

Comparison parameter of means and variation for germination of different chemical scarification treatment.

Chemical Treat	Generation percentage in a week (GPW ± SD)	Generation percentage (GP ± SD)	Generation energy (GE ± SD)	Mean germination time (MGT ± SD)	Germination index (GI ± SD)	Germination rate index (GRI ± SD)	No. of seedling secondary roots (SSR ± SD)	No. of seedling cotyledon (SC ± SD)
Control (NC)	22.67^b ± 4.73	25.33^b ± 4.04	6.33^b ± 1.01	6.00^ab ± 0.93	277.67^b ± 43.14	4.41^bc ± 0.69	23.33^ab ± 2.52	1.00^ab ± 0.00
GA3	8.67^cde ± 2.08	9.00^de ± 2.00	2.25^de ± 0.50	4.46^bc ± 0.39	112.67^cd ± 24.71	2.12^de ± 0.45	8.33^c ± 1.53	1.00^ab ± 0.00
NaHClO3	57.33^a ± 7.51	92.00^a ± 3.00	23.00^a ± 0.75	7.25^a ± 0.48	897.00^a ± 63.15	15.30^a ± 1.53	34.33^a ± 12.70	1.50^a ± 0.71
H2SO4	22.67^b ± 2.89	26.33^b ± 2.08	6.58^b ± 0.52	5.39^bc ± 0.52	305.33^b ± 22.72	5.45^b ± 0.47	23.33^ab ± 2.52	0.50^ab ± 0.71
KNO3	17.00^bc ± 2.00	19.67^c ± 0.58	4.92^c ± 0.14	5.35^bc ± 0.89	229.33^b ± 22.59	4.18^bc ± 0.69	13.33^bc ± 1.53	1.00^ab ± 0.00
KCl	11.67^cd ± 2.52	12.00^d ± 2.00	3.00^d ± 0.50	4.89^bc ± 0.53	146.00^c ± 30.61	2.72^cd ± 0.71	9.67^c ± 3.06	0.50^ab ± 0.71
PEG	4.00^de ± 1.00	4.33^ef ± 1.53	1.08^ef ± 0.38	5.72^abc ± 0.86	48.00^de ± 13.12	0.78^e ± 0.17	3.33^c ± 0.58	0.00^b ± 0.00
NaCl	1.33^f ± 0.58	1.33^f ± 0.57	0.33^f ± 0.14	4.17^c ± 0.29	17.00^e ± 6.93	0.32^e ± 0.12	1.33^c ± 0.58	0.00^b ± 0.00

Control (NC) is a nonchemical treatment: 200 ppm gibberellic acid (GA₃) for 30 min, sodium hypochlorite (NaHClO₃) for 30 min, 2% (v/v) sulfuric acid (H₂SO₄) for 10 min, 150 mM potassium nitrate (KNO₃) for 30 min, 2% (w/v) potassium chlorite (KCl) for 10 min, and 10% polyethylene glycol (PEG) for 24 h and 25 mM sodium chloride (NaCl) for 24 h.