Determination of Mealybug Population Density for Transmission of Pineapple Mealybug Wilt Disease in Central Uganda

Figure 3

PCR amplification of the viruliferous mealybug subpopulation used for inoculating pineapple plants at the screenhouse, Faculty of Agriculture, Kyambogo University, 2017 and 2018. Note. M is the DNA gene ruler, lanes 1–7 are PCR-amplified samples using primer set 225/226, lane marked (+) is the positive control, and lane marked (−) is the negative control. Subpopulation from sample 5 was used for pineapple plant inoculation.