Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4-sulphonate

Table 4

Recovery studies for the proposed spectrofluorometric method for the determination of AMD in spiked human plasma.

Spiked concentration (𝜇g mL^−1)	Recovery (%±RSD)^(a)
	Intra-assay	Inter-assay
0.05	98.2±2.04	96.3±4.20
0.10	99.3±1.01	101.1±2.0
0.20	100.5±0.99	99.1±1.50
0.40	100.9±0.74	98.5±1.52
0.80	100.1±0.75	101.2±1.44
1.60	98.6±0.82	100.3±1.12
3.20	98.9±0.57	99.7±0.72

^(a)Values are mean of three and five determinations for intra- and inter-assay, respectively.