Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4-sulphonate

Table 5

Analysis of AMD in capsules by the proposed and official methods.

Method	Recovery (%±SD)^(a)	𝑡-value^(b)	𝐹-value^(b)
Spectrophotometric	98.70±1.79	0.42	1.64
Spectrofluorometric	98.91±1.91	0.19	1.85
Official HPLC^(c)	99.13±1.41	—	—

^(a)Values are mean of five determinations.^(b)Theoretical values for 𝑡 and 𝐹 at 95% confidence limit and 𝑛=5 were 2.31 and 6.39, respectively.^(c)Reference [32].