A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup

Table 4

Limits of detection (LOD) and quantitation (LOQ) of the HPLC method for the determination of hederacoside C in Ivy-Thyme cough syrup.

Standard deviation of the response (SD^∗)	LOD^a (mg/mL)	LOQ^a (mg/mL)
14831.8	0.011	0.032

∗The value of SD was calculated as the standard deviation of 𝑦 intercepts of regression lines plotted, at the chromatographic responses of the calibration solutions (0.03–0.15 mg/mL), parallel to the calibration curve (slope=4612.8).
aFor calculation of LOD and LOQ values, see Results and Discussion.