Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching

Figure 6

Quenching efficiency (𝜃) versus concentration of quencher: (a) 2.5 mg/mL E2-bound NIP particles in aqueous suspension quenched with AuNPs, (b) 2.5 mg/mL E2-MIP particles in aqueous suspension quenched with AuNPs, (c) 3.5 ppm E2 aqueous solution quenched with AuNPs, and (d) 2.5 mg/mL E2-MIP particles in aqueous suspension quenched first with AuNPs and next with sodium nitrite. (e) 2.5 mg/mL E2-MIP particles in aqueous suspension quenched with sodium nitrite (up to 0.128 M).