Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching

Scheme 1

Larger AuNPs were used in the first step of fluorescence quenching to quench E2 molecules nonspecifically bound throughout the porous MIP particle while small nitrite anions easily penetrate the MIP particle to quench the fluorescence emission from E2 molecules specifically bound inside imprinted cavities in the second step of fluorescence quenching. The attenuated fluorescence emission intensity between step 1 and step 2 varies as a function of E2 concentration.