International Journal of Analytical Chemistry / 2011 / Article / Tab 1

Research Article

Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities

Table 1

Activities (U/mL or U/g) of different enzyme preparations toward CMC, barley β-glucan, birchwood glucuronoxylan, and galactomannan measured using the DNS and NS assays.

No.CMCaseβ-GlucanaseXylanaseβ-Mannanase
DNSNSDNS/NSDNSNSDNS/NSDNSNSDNS/NS*DNSNSDNS/NS

(1)1311121.250495449.312153533.4 (5.5)329369.1
(2)282123821.235624305111.7838128672.9 (5.7)
(3)119861.4673798.5345883.9 (8.0)118167.4
(4)3742671.4327528811.413804902.8 (11.5)359409.0
(5)599542461.431915262812.1393311733.4 (8.4)
(6)71501.421712249.710291735.9 (8.8)202336.1
(7)124831.56921175.916945003.4 (5.8)96118.7
(8)127851.51177091612.818905073.7 (5.8)386765.1
(9) 63411.56366210.313792834.9 (8.5)106157.1
(10)1175576071.571920628411.474350244173.0 (11.2)
(11)947458761.660611580610.4321904586005.5 (10.4)9821576.3
(12)132791.735914697.79081615.6 (8.1)10401457.2

Average ratio1.410.14.0 (8.1)7.3

Std. deviation0.22.01.1 (2.1)1.4

*Ratio of xylanase activities determined with arabinoxylan from wheat as a substrate is given in parentheses.

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